HPLC波长切换法同时测定爵床中5个黄酮类成分的含量

目的:建立HPLC切换波长的方法同时测定爵床中山柰酚-3-O-[2-O-β-D-吡喃葡萄糖基-6-O-α-L-吡喃鼠李糖基]-β-D-吡喃葡萄糖苷、山柰酚-3-O-[2-O-α-L-吡喃鼠李糖基-6-O-β-D-吡喃木糖基]-β-D-吡喃葡萄糖苷、山柰酚-3-O-β-D-芸香糖苷、槲皮素和山柰酚的含量。方法:采用Agilent Eclipse XDB-C₁₈(4.6 mm×150 mm,5 μm)色谱柱,以乙腈-0.05%磷酸水溶液为流动相,梯度洗脱,流速0.7 mL·min^-1,检测波长267 nm(0~55 min)、370 nm(55.1~85 min),柱温25 ℃。结果:山柰酚-3-O-[2-O-β-D-吡喃葡萄糖基-6-O-α-L-吡喃鼠李糖基]-β-D-吡喃葡萄糖苷、山柰酚-3-O-[2-O-α-L-吡喃鼠李糖基-6-O-β-D-吡喃木糖基]-β-D-吡喃葡萄糖苷、山柰酚-3-O-β-D-芸香糖苷、槲皮素、山柰酚的进样量分别在0.049~0.389 μg(r=0.999 2)、0.052~0.416 μg(r=0.999 6)、0.068~0.547 μg(r=0.999 7)、0.041~0.326 μg(r=0.999 8)和0.056~0.450 μg(r=0.999 7)范围内,与色谱峰面积呈良好的线性关系;平均回收率(n=6)分别为100.6%、98.1%、100.7%、98.2%、102.5%。不同来源的爵床药材中5个成分的含量差异明显。结论:本法操作简单、可靠,重复性好,可为爵床的质量评价提供参考依据。

Objective:To establish a method of wavelength switching for the simultaneous determination of contents of 5 flavonoids,kaempferol-3-O-[2-O-β-D-glucopyransyl-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside,kaempferol-3-O-[2-O-α-L-rhamnopyranosyl-6-O-β-D-xylopyranosyl]-β-D-glucopyranoside,kaempferol-3-O-β-D-rutinoside,quercetin,and kaempferol in Herba Rostellulariae.Methods:The agilent Eclipse XDB-C₁₈ column(4.6 mm×150 mm,5 μm)was adopted.The mobile phase was acetonitrile(A)-0.05% phosphoric acid aqueous solution(B)with gradient elution at a flow rate of 0.7 mL·min^-1.The detection wavelength was 267 nm(0-55 min)and 370 nm(55.1-85 min).The column temperature was 25 ℃.Results:The method achieved a good linearity in the ranges of 0.049-0.389 μg(r=0.999 2)for kaempferol-3-O-[2-O-β-D-glucopyransyl-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside,0.052-0.416 μg(r=0.999 6)for kaempferol-3-O-[2-O-α-L-rhamnopyranosyl-6-O-β-D-xylopyranosyl]-β-D-glucopyranoside,0.068-0.547 μg(r=0.999 7)for kaempferol-3-O-β-D-rutinoside,0.041-0.326 μg(r=0.999 8)for quercetin,and 0.056-0.450 μg(r=0.999 7)for kaempferol;the average recoveries(n=6)were 100.6%,98.1%,100.7%,98.2%,102.5%,respectively.The contents of five constituents in Rostellularia from different sources had great differences.Conclusion:This method is simple,accurate,reproducible,and convenient for the quality control of Herba Rostellulariae.