大黄素对急性胰腺炎大鼠相关炎性因子表达的影响

目的:观察大黄素对重症急性胰腺炎(SAP)大鼠模型肠屏障功能保护作用机理。方法:雄性SD大鼠45只,随机(随机数字法)分为3组:假手术组(SO组)、重症急性胰腺炎组(SAP)、大黄素处理组(E组),每组15只。采用5%牛磺胆酸钠逆行胆胰管注射制备大鼠SAP模型,E组在造模后30 min大黄素灌胃(20 mg·kg^-1),术后3、6、12 h分批剖杀大鼠,各时间点5只。检测血清肿瘤坏死因子(TNF)-α、白介素(IL)-6、二胺氧化酶(DAO)水平,光镜观察胰腺组织病理变化,RT-PCR检测外周血单核细胞(PBMCs)Toll样受体4(TLR4)mRNA表达水平。多个样本均数比较采用单因素方差分析,两组间比较采用t检验。结果:SAP组及E组均造模成功。SAP组、E组与SO组比较,胰腺组织病理学评分、单核细胞表面TLR4表达、血清TNF-α、IL-6、DAO水平均显著升高(P < 0.05);与SAP组比较,E组胰腺组织病理学评分、TLR4mRNA表达、TNF-α、IL-6、DAO水平均显著降低(P < 0.05)。结论:大黄素治疗可通过下调TLR4mRNA表达,明显改善SAP肠屏障功能。

Objective:To observe the effects of emodin on intestina1 barrier function in rats with severe acute pancreatitis(SAP),and explore its mechanism.Methods:Forty-five male Sprague-Dawley rats were randomly(random number)divided into sham operation(SO)group,severe acute pancreatitis(SAP)group and emodin treatment group(n=15 in each group).SAP rat model was established by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct.Rats in emodin group were then lavaged with emodin(20 mg·kg^-1)after the modeling.Five rats in each group were sacrificed at 3,6,12 hours,respectively after modeling.The serum levels of amylase,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and diamine oxidase(DAO)were detected,and pathological scores of the tissue of pancreas were evaluated under light microscope.TLR4 mRNA expressions on PBMCs were detected by RT-PCR.All data were analyzed by using the analysis of variance or t test.Results:The pancreatitis models were established successfully in group SAP and E.Compared with those in group SO,pancreatic pathological scores,levels of TLR4mRNA,TNF-α,IL-6 and DAO in group SAP and E were significantly increased(P < 0.05).Pancreatic pathological score,levels of TLR4mRNA,TNF-α,IL-6 and DAO in group E were significantly decreased compared with those in group SAP(P < 0.05).Conclusion:Emodin can significantly down-regulate the expressions of TLR4 mRNA and obviously improve the severity of SAP.