蒙药白益母草定性定量方法研究

目的: 建立白益母草定性定量方法,以多指标成分综合评价白益母草质量。方法: 采用化学和薄层色谱法对白益母草中黄酮类化合物进行定性鉴别;运用高效液相色谱法对其进行定量分析,采用Inertsil ODS-SP (250 mm×4.6 mm,5 μm)色谱柱,以乙腈-水为流动相梯度洗脱,检测波长为261 nm,柱温为35 ℃。结果: 白益母草供试品溶液盐酸-镁粉反应阳性,灵敏度高;薄层鉴别斑点清晰,重现性好;HPLC分析,异鼠李素-3-O-芸香糖苷(S1)、5,6,7,4'-四羟基黄酮-7-O-(6"-O-[E]-咖啡酰基)-葡萄糖苷(S2)、5,6,7,4'-四羟基黄酮-7-O-(6"-O-[E]-香豆酰基)-葡萄糖苷(S3)、异鼠李素-3-O-葡萄糖苷(S4)、金丝桃苷(S5)、芹菜素-7-O-葡萄糖苷(S6)和7,4'-二甲氧基芹菜素(S7)质量浓度分别在100.00~500.00(r=0.999 7)、20.00~100.00(r=0.999 9)、10.00~50.00(r=0.999 9)、5.00~25.00(r=0.999 5)、2.00~10.00(r=0.999 5)、1.00~5.00 (r=0.999 7)和1.00~5.00 μg·mL^-1(r=0.999 4)范围内具有良好的线性关系,平均回收率(n=6)分别为98.67%(RSD=1.20%)、97.42%(RSD=1.45%)、98.32%(RSD=2.08%)、96.85%(RSD=1.27%)、97.23%(RSD=1.95%)、96.37%(RSD=1.40%)、96.54%(RSD=1.63%),8批样品中上述7种成分平均含量范围分别为5142.0~5714.0、1332.0~1465.0、724.0~802.0、302.4~330.8、113.4~132.4、58.69~62.08、58.75~62.57 μg·g^-1。结论: 通过实验研究,建立了白益母草定性和定量分析方法,为药材质量检测提供依据。

[1] ZHU YM(朱亚民).Flora of Inner Mongolia(内蒙古植物药志)[M].Huhehaote(呼和浩特):Inner Mongolia People's Press(内蒙古人民出版社), 1993:464
[2] ZOU SX(邹淑仙), BAO LR (宝乐日).Study on the extraction and isolation of flavonoids from Panzeria alashanica(天然植物脓疮草中黄酮类有效成分提取与分离的研究)[J].J Inner Mongolia Univ(内蒙古大学学报), 2001, 32(1):50
[3] HOU FF(侯风飞), ZHENG YF(郑亚夫), ZHANG HM(张海鸣), et al.Study on chemical constituents from Panzeria alashanica(蒙药白益母草的化学成分研究)[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2009, 15(9):17
[4] LI XL(李雪丽), ZHANG MF(张美锋), ZHU D(朱丹).Extraction and determination of alkaloid in Mongolian herba Panzeria alaschanica(蒙药白益母草总生物碱的提取分离及含量测定)[J].China J Trade Chin Med(中华中医药杂志), 2011, 26(9):2162
[5] MARTORANA M, ARCORACI T, RIZZA L, et al.In vitro antioxidant and in vivo photoprotective effect of pistachio (Pistacia vera L., variety Bronte) seed and skin extracts[J]. Fitoterapia, 2013, 85(10):41
[6] FOURIE TG, SNYCKERS FO.A flavone with anti-inflammatory activity from the roots of Rhus undulata[J].J Nat Prod, 1984, 47(6):1057
[7] PENG ZF, STRACK D, BAUMERT A, et al.Antioxidant flavonoids from leaves of Polygonum hydropiper L.[J].Phytochemistry, 2003, 62(2):219
[8] SILVA GN, MARTINS FR, MATHEUS ME, et al.Investigation of anti-inflammatory and antinociceptive activities of Lantana trifolia[J]. J Ethnopharmacol, 2005, 100(3):254
[9] HASAN K, ETIL A, MILENA M, et al.Iridoid, phenylethanoid and flavonoid glycosides from Sideritis trojana[J].Fitoterapia, 2012, 83(1):130
[10] ALBACHA DC, GRAYERB RJ, JENSEN SR, et al.Acylated flavone glycosides from Veronica[J].Phytochemistry, 2003, 64(7):1295
[11] MENG YH, KRZYSIAK AJ, DURAKO MJ, et al.Flavones and flavone glycosides from Halophila johnsonii[J].Phytochemistry, 2008, 69(14):2603
[12] JULIÃO LDS, LEITÃO SG, LOTTI C, et al.Flavones and phenylpropanoids from a sedative extract of Lantana trifolia L.[J].Phytochemistry, 2010, 71(2/3):294