独一味的HPLC指纹图谱研究

目的: 建立独一味的指纹图谱分析方法用于该药材的质量评价。方法: 采用HPLC法,选用Welchrom C₁₈色谱柱(250 mm×4.6 mm,5 μm),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱(0~10 min,5%A→8%A;10~27 min,8%A→12%A;27~35 min,12%A;35~70 min,12%A→17%A;70~80 min,17%A→19%A;80~110 min,19%A→36%A;110~120 min,36%A→50%A),流速1.0 mL·min^-1,检测波长235 nm,柱温30℃;用国家药典委员会"中药色谱指纹图谱相似度评价系统2004A"处理分析。结果: 对23批独一味地上部分样品指纹图谱进行评价,确定了14个共有峰,指认了第3、4、5、8、9、10、11、12号色谱峰分别对应为胡麻属苷、山栀苷甲酯、绿原酸、马钱苷、8-O-乙酰山栀苷甲酯、连翘酯苷B、木犀草苷和麦角甾苷。结论: 本研究可更全面、准确地评价独一味药材质量,为该药材的质量控制提供科学依据。

Objective: To establish a fingerprint analysis method for quality evaluation of Lamiophlomis rotata. Methods: HPLC was employed,and the separation was performed on a Welchrom-C₁₈ chromatographic column (250 mm×4.6 mm,5 μm)using acetonitrile(A)-0.1% orthophosphoric acid(B)as mobile phase with gradient elution(0-10 min,5%A→8%A;10-27 min,8%A→12%A;27-35 min,12%A;35-70 min,12%A→17%A;70-80 min,17%A→19%A;80-110 min,19%A→36%A;110-120 min,36%A→50%A)at a flow rate of 1.0 mL·min^-1.The detection wavelength was set at 235 nm and the column temperature was controlled at 30℃.The data were analyzed with similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(2004A). Results: The total 23 batches of aerial part of Lamiophlomis rotata were evaluated,and fourteen common chromatographic peaks were observed.Then,the No.3,No.4,No.5,No.8,No.9,No.10,No.11 and No.12 peaks were identified as sesamoside,shanzhiside methylester,chlorogenic acid,loganin,8-O-acetyl shanzhiside methylester,forsythiaside B,luteolin glycosides and acteoside,respectively. Conclusion: The established method is more comprehensive and accurate in evaluating the quality of Lamiophlomis Herba and can provide the scientific basis for quality control.