UPLC-MS/MS法同时测定白芍饮片中10种成分

目的: 建立超高效液相色谱串联质谱法(UPLC-MS/MS法)同时测定白芍饮片中10种主要成分(没食子酸、原儿茶酸、芍药苷亚硫酸酯、氧化芍药苷、儿茶素、没食子酸甲酯、芍药内酯苷、芍药苷、五没食子酰葡萄糖、苯甲酰芍药苷)的含量。方法: 采用UPLC-MS/MS法,负离子多反应监测(MRM)模式进行含量测定。色谱条件:采用Waters CORTECS C₁₈(2.1 mm×100 mm,1.6 μm)色谱柱,以乙腈(A)-0.1%甲酸水(B)为流动相,梯度洗脱,流速0.25 mL·min^-1,柱温45 ℃。结果: 在所设定的色谱条件下,5 min内定量分析白芍中10种主要成分在考察的浓度范围内呈良好的线性关系(r>0.996 6);回收率和RSD分别在97.3%~102.0%和3.1%~5.1%范围内。结论: 本研究所建立的同时测定白芍中10种主要成分(没食子酸,原儿茶酸,芍药苷亚硫酸酯,氧化芍药苷,儿茶素,没食子酸甲酯,芍药内酯苷,芍药苷,五没食子酰葡萄糖,苯甲酰芍药苷)的UPLC-MS/MS定量分析方法简便、快捷、准确,为白芍饮片质量控制提供新的技术手段。

Objective: To establish a rapid,accurate UPLC-MS/MS method for the determination of 10 major components(gallic acid,potocatechuic acid,paeoniflorin sulfonate,oxypaeoniflorin,catechin,methyl gallate,albiflorin,paeoniflorin,pentagalloylglucose,benzoylpaeoniflorin)in Radix Paeoniae Alba pieces. Methods: The UPLC-MS/MS assay was performed on a Waters CORTECS C₁₈(2.1 mm×100 mm,1.6 μm)column with acetonitrile-water(containing 0.1% formic acid)as the mobile phase by gradient elution at a flow rate of 0.25 mL·min^-1.The column temperature was set at 45 ℃.MS detection was performed with multiple reaction monitoring(MRM)mode using negative electrospray ionization. Results: Under the optimized chromatographic conditions,good separation of 10 target components were obtained within 5 min.Satisfactory linearity was achieved within the investigated linear range and with fine determination coefficient(r>0.996 6),the overall recoveries were ranged from 97.3%-102.0% with the RSD ranging from 3.1%-5.1%. Conclusion: It is the first report of simultaneous analysis of 10 major components(gallic acid,potocatechuic acid,paeoniflorin sulfonate,oxypaeoniflorin,catechin,methyl gallate,albiflorin,paeoniflorin,pentagalloylglucose,benzoylpaeoniflorin)in Radix Paeoniae Alba by using UPLC-MS/MS method,which affords a highly sensitive,specific,speedy and efficient method for quality control of Radix Paeoniae Alba pieces.