UPLC法测定郁舒片中5种药效成分含量

目的: 建立UPLC法同时测定郁舒片中芍药内酯苷、芍药苷、芦丁、金丝桃苷、异槲皮苷的含量. 方法: 采用Waters Acquity UPLC^TM BEH-C₁₈(100 mm×2.1 mm,1.7 μm)色谱柱,以0.1%磷酸水溶液(A)-乙腈(B)为流动相,梯度洗脱,流速0.4 mL·min^-1,检测波长203 nm,柱温30℃. 结果: 芍药内酯苷、芍药苷、芦丁、金丝桃苷、异槲皮苷5种成分在相应的范围内,呈 良好的线性关系(r≥0.999 0);方法的平均加样回收率(n=6)分别为101.4%(RSD=0.82%)、101.9%(RSD=1.0%)、 99.0%(RSD=2.6%)、98.9%(RSD=2.0%)、100.0%(RSD=2.4%).3批样品的测定结果分别是芍药内酯苷3.53~3.80 mg·片^-1,芍药苷9.33~10.01 mg·片^-1,芦丁0.89~0.98 mg·片^-1,金丝桃苷0.72~0.76 mg·片^-1,异槲皮苷0.82~0.86 mg·片^-1. 结论: 该方法分析时间短,操作简单,可以作为郁舒片的质量控制方法.

Objective: To establish a UPLC method for the simultaneous determination of five effective components (albiflorin,paeoniflorin,rutin,hyperin,and isoquercitrin) in Yushu tablets. Methods: The analysis was performed on a Waters Acquity UPLC system with a Waters ACQUITY UPLC^TMBEH C₁₈ column (100 mm×2.1 mm,1.7 μm).The five effective components were separated with a gradient mobile phase consisting of 0.1% phosphoric acid aqueous(A)-acetonitrile(B) at a flow rate of 0.4 mL·min^-1,and the UV detection wavelength was set at 203 nm,the column temperature was 30℃. Results: The contents of albiflorin,paeoniflorin,rutin,hyperin,and isoquercitrin in the corresponding linear ranges showed good linear relationships (r≥0.999 0);the average recoveries (n=6) of the five components were 101.4% (RSD=0.82%),101.9% (RSD=1.0%),99.0% (RSD=2.6%),98.9% (RSD=2.0%),100.0% (RSD=2.4%),respectively.In the 3 batches of samples,the contents of albiflorin,paeoniflorin,rutin,hyperin,and isoquercitrin were in the ranges of 3.53-3.80,9.33-10.01,0.89-0.98,0.72-0.76,and 0.82-0.86 mg per tablet. Conclusion: The developed method is time-saving and easy to operate,which can be used as a method of quality control of Yushu tablets.