HPLC法同时测定六味能消胶囊中木香烃内酯、去氢木香烃内酯大黄素、大黄酚的含量

目的:建立六味能消胶囊中木香烃内酯、去氢木香烃内酯、大黄素、大黄酚4个成分含量测定的方法。方法: 采用Atlantis C₁₈ 色谱柱(150 mm×4.6 mm,5 μm),以甲醇-0.1%磷酸水为流动相,梯度洗脱,检测波长225 nm(0~25 min检测木香烃内酯、去氢木香烃内酯)、254 nm(25~40 min检测大黄素、大黄酚),柱温25 ℃。结果: 木香烃内酯、去氢木香烃内酯、大黄素、大黄酚的线性范围分别为0.665 6~33.28、0.563 7~31.84、0.524 8~26.24、0.585 6~29.28 μg · mL^-1;平均回收率(n=6)分别为99.28%(RSD=1.8%),101.7%(RSD=1.6%),101.2%(RSD=2.0%),99.36%(RSD=1.8%)。5批样品中木香烃内酯、去氢木香烃内酯、大黄素、大黄酚含量分别为2.32~2.41、2.48~2.62、0.42~0.48、1.26~1.33 mg · g^-1。结论: 该方法可用于六味能消胶囊中木香烃内酯、去氢木香烃内酯、大黄素、大黄酚的同时测定。

Objective: To develop an HPLC method for the determination of costunolide, dehydrocostus lactone, emodin, and chrysophanol in Liuweinengxiao capsules. Methods: Samples were analyzed on an Atlantis C₁₈ column(150 mm×4.6 mm, 5 μm)eluted with methanol and water containing 0.1% phosphate acid as mobile phases in a linear gradient mode.The flow rate was kept at 1.0 mL · min^-1, and the column temperature was set at 25 ℃.The detector wavelengths were 225 nm(0-25 min)for costunolide and dehydrocostus lactone, 254 nm(25-40 min)for emodin and chrysophanol. Results: The linear ranges of costunolide, dehydrocostus lactone, emodin and chrysophanol were 0.665 6-33.28 μg · mL^-1, 0.563 7-31.84 μg · mL^-1, 0.524 8-26.24 μg · mL^-1, 0.585 6-29.28 μg · mL^-1, respectively.The average recoveries(n=6)of the four constituents were 99.28%(RSD=1.8%), 101.7%(RSD=1.6%), 101.2%(RSD=2.0%), 99.36%(RSD=1.8%), respectively.The contents of the four constituents were 2.32-2.41 mg · g^-1, 2.48-2.62 mg · g^-1, 0.42-0.48 mg · g^-1, and 1.26-1.33 mg · g^-1. Conclusion: The established method can be used for simultaneous determination of costunolide, dehydrocostus lactone, emodin and chrysophanol in Liuweinengxiao capsules.