期刊名称:药物分析杂志 主管单位:中国科学技术协会 主办单位:中国药学会承办:中国食品药品检定研究院 主编:金少鸿 地址:北京天坛西里2号 邮政编码:100050 电话:010-67012819,67058427 电子邮箱:ywfx@nifdc.org.cn 国际标准刊号:ISSN 0254-1793 国内统一刊号:CN 11-2224/R 邮发代号:2-237
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松木层孔菌杂多糖中的3-O-甲基-半乳糖的分析鉴定
Identification of 3-O-methyl-galactose in heteropolysaccharide isolated from the fruiting body of Phellinus pini
分类号:
出版年·卷·期(页码):2015,35 (2):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
目的: 分析鉴定松木层孔菌子实体杂多糖中的未知单糖组分。方法: 松木层孔菌子实体经提取分离纯化得到水溶性均一多糖组分PP80W,HPLC法测定其均一性和分子量。PP80W经水解、还原和乙酰化后采用GC法对照测定其单糖组分,并对其中未知的1个单糖组分采用GC-MS、DEPT-135和2D NMR(1H-1H COSY、TOCSY、HMQC、HMBC和NOESY谱)进一步分析鉴定。结果: PP80W相对分子质量为2.99×103,GC分析发现PP80W由鼠李糖、甘露糖、葡萄糖、半乳糖和1种未知单糖构成,GC-MS和核磁共振分析鉴定该未知单糖为3-O-甲基-半乳糖。结论: PP80W为含有3-O-甲基-半乳糖结构的杂多糖组分。
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Objective: To identify an unknown sugar component in polysaccharide PP80W purified from the fruiting body of Phellinus pini. Methods: A water-soluble and mono-disperse polysaccharide PP80W was obtained from the fruiting body of Phellinus pini by hot water extraction, separation and purification.The homogeneity and molecular weight of PP80W were analyzed by HPLC.PP80W was hydrolyzed with TFA, reduced with NaBH4 and acetylated with acetic anhydride.Then the monosaccharide composition of PP80W was analyzed by GC.GC-MS, DEPT-135 and 2D NMR(1H-1H COSY, TOCSY, HMQC, HMBC and NOESY spectra)were further used to identify the unknown sugar component. Results: PP80W, with a relative molecular mass of 2.99×103, was determined to be composed of rhamnose, mannose, glucose, galactose and an unknown monosaccharide by GC analysis.The unknown monosaccharide was identified as 3-O-methyl-galactose by GC-MS and NMR analysis. Conclusion: PP80W was a heteropolysaccharide contain 3-O-methyl-galactose.
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