碘仿原位凝胶的含量测定方法建立

目的: 建立紫外分光光度法测定碘仿原位凝胶的含量.方法: 以乙醇为溶剂,在341 nm波长下测定碘仿原位凝胶的含量.通过精密度试验、回收率试验、稳定性试验等方法学研究,建立紫外分光光度法测定碘仿原位凝胶的含量.结果: 碘仿测定的线性方程为Y=0.0065X+0.007(r=0.9993),检测浓度在35.6~119.6 μg·mL^-1范围内与吸光度具有良好的线性关系,平均回收率(n=9)为100.2%.结论: 建立的方法可用于碘仿原位凝胶的含量检测.

Objective: To establish a method for the determination of iodoform in-situ gel by ultraviolet spectrophotometry. Methods: The contents of iodoform in-situ gel were determined at 341 nm with ethanol as the solvent by ultraviolet spectrophotometry.The method for determination of iodoform in-situ gel was achieved through the investigation of precision, recovery and stability by ultraviolet sepectrophotometry. Results: The linear equation was Y=0.0065X+0.007(r=0.9993), and the calibration curve of iodoform was linear when its concentration range was 35.6-119.6 μg·mL^-1(r=0.9993).The average recovery rate(n=9)was 100.2%. Conclusion: The method can be used for determination of iodoform in-situ gel.

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