纳洛酮对酒精所致小鼠急性脑损伤和肝损伤的保护作用

目的: 观察纳洛酮对小鼠急性酒精性脑损伤和肝损伤的保护作用.方法: 将昆明种小鼠分为对照组、酒精组、纳洛酮组.纳洛酮组腹腔注射纳洛酮,30 min后,酒精组和纳洛酮组灌胃给予56°酒精,造成急性酒精性脑损伤和肝损伤模型,观察并记录小鼠翻正反射消失时间和动物数.2 h后处死小鼠,立即取出脑和肝,制备脑匀浆和肝匀浆,按试剂盒要求检测肝和脑中丙二醛(MDA)、谷胱甘肽(GSH)、钙离子(Ca²⁺)含量和一氧化氮合酶(NOS)活性.结果: 与酒精组比较,纳洛酮组小鼠入睡时间明显延长,翻正反射消失动物数减少.与空白组相比,酒精组脑组织和肝组织MDA含量、NOS活性和Ca²⁺含量升高,GSH的含量减少.纳洛酮组与酒精组比较,脑组织和肝组织MDA含量、Ca²⁺含量、NOS活性降低,GSH的含量升高.结论: 纳洛酮能对抗并清除自由基,降低小鼠脑组织和肝组织MDA含量、Ca²⁺含量和NOS活性,提高GSH的含量,对酒精性脑损伤和肝损伤有保护作用.

Objective: To observe the protective effect of naloxone against acute brain and liver injury induced by alcohol in mice. Methods: Mice were divided into control group, alcohol group and naloxone group.The mice in naloxone group received intraperitoneal injection of naloxone.After 30 minutes, the acute brain and liver injury model was induced by intragastric administration of 56° alcohol in alcohol and naloxone groups.The time and the animal number of righting reflex loss in mice were recorded.Mice were killed after 2 h, and the brain and liver were removed for preparation of brain and liver homogenates, and the activity of NOS and the contents of MDA, GSH, Ca²⁺ were detected in the homogenates with test kit. Results: The sleep latency was significantly prolonged and the animal number of righting reflex loss was decreased in naloxone group compared with control group.The contents of MDA and Ca²⁺ and the activity of NOS significantly increased and the content of GSH significantly decreased in brain and liver tissues in alcohol group compared with control group.Compared with alcohol group, the contents of MDA and Ca²⁺ and the activity of NOS was decreased and the contents of GSH was increased in brain and liver tissues in naloxone group. Conclusion: Naloxone can protect against alcoholic brain and liver injury by scavenging free radicals, increasing the GSH content, decreasing the contents of MDA and Ca²⁺ and the activity of NOS in brain and liver.