金莲花的薄层鉴别与含量测定研究

目的: 建立金莲花的薄层鉴别与含量测定方法。方法: 比较甲醇、乙醇、70%乙醇等不同提取溶剂、不同展开系统在硅胶G板上的分离效果,建立金莲花的薄层色谱鉴别方法;比较不同溶剂的提取效果、不同流动相的分离效果,流速1.0 mL·min^-1,检测波长340 nm,ODS色谱柱柱温30 ℃,建立HPLC法测定金莲花中荭草苷、牡荆素的含量。结果: 建立的TLC法能很好分离各成分,HPLC法能够准确测定金莲花中荭草苷、牡荆素的含量。结论: 本文所建立的TLC、HPLC法可用于金莲花质量检测。

Objective: To establish methods of the TLC identification and the content determination of Trollii Chinensis Flos. Methods: The separation effects by different extraction solvents such as methanol,ethano l,70% ethanol and different deployment systems on the silica gel G plate were compared to establish a TLC identification method for Trollii Chinensis Flos.The extraction effects of different solvents and the separation effects of different mobile phases were compared,and the content determination method of HPLC for orientin and vitexin in Trollii Chinensis Flos was established using the ODS column,and the flow rate was 1 mL·min^-1,the UV detection wavelength was 340 nm,the column temperature was 30 ℃. Results: The established TLC method had a good separation effect for each component,the established HPLC method can accurately determine the content of orientin and vitexin in Trollii Chinensis Flos. Conclusion: The established methods of TLC and HPLC can be used to test the sample of Trollii Chinensis Flos.

[1] ChP 1977.Vol Ⅰ(中国药典1978年版.一部)[S].1978:359
[2] ChP 2010.Vol Ⅰ(中国药典2010年版.一部)[S]2010:549
[3] NAN Min-lun(南敏伦),ZHAO Yu-wei(赵昱玮),SI Xue-ling(司学玲),et al.Study on dynamic changes of three flavonoid C-glycosides constituents in different harvest time of trollius chninesis(不同采收期金莲花中3中碳苷黄酮成分动态变化)[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2013,19(4):118
[4] YANG Jing-fan(杨晶凡),ZHANG Chun-hui(张春辉),HAN Ru(韩茹),et al.The determination of four active compounds alignment in different storage period of Flos Trollii Chinensis by HPLC(不同储存期金莲花中4种药效组分的测定)[J].Mod Chin Med(中国现代中药),2009,11(1):16
[5] TAN An-ju(谭安菊),ZHOU Wen(周雯),CHEN Hui(陈慧),et al.HPLC fingerprint analysis of Trollius chinensis(金莲花药材高效液相色谱指纹图谱研究)[J].J Guiyang Med Coll(贵阳医学院学报),2013,38(5):489
[6] SONG Dong- mei(宋冬梅),SUN Qi-shi(孙启时).Chemical studies on the constituents of Trollius altaicus C.A.Mey.(阿尔泰金莲花化学成分的研究(I))[J].Chin J Med Chem(中国药物化学杂志),2004,14(4):233
[7] WEI Jin-xia(魏金霞),LI Dan-yi(李丹毅),HUA Hui-ming(华会明),et al.Isolation and identification of chemical constituents from flowers of Trollius chinensis(Ⅱ)(金莲花化学成分的分离与鉴定(Ⅱ))[J].J Shenyang Pharm Univ(沈阳药科大学学报),2012,29(1):12
[8] WEN Rui-qing(温瑞卿),ZHANG Gui-jun(张贵军),YANG Yang(杨洋).Chromatographic identification of medicinal components orientin and vitexin in Trollius chinensis(金莲花中药效成分荭草苷和牡荆苷的色谱鉴定)[J].World Notes Plant Med(国外医药·植物药分册),2008,23(5):214