实时直接分析质谱法快速筛查葡萄酒中9种甜味剂

目的:建立一种实时直接分析质谱法快速筛查葡萄酒中安赛蜜、阿斯巴甜、纽甜、甘草次酸、麦芽糖醇、异麦芽酮糖、木糖醇、D-山梨醇、D-甘露醇共9种甜味剂。方法:实时直接分析离子源串联三重四极杆质谱,源电压为350 V,载气为氦气,源温度350 ℃,样品传输速度为2 mm·s^-1,采用子离子扫描模式直接分析葡萄酒样品,通过对照品与样品的二级质谱图对比进行非法添加成分的定性鉴别。结果:9种甜味剂检出限为2.5~20 μg·mL^-1,5批样品中检出麦芽糖醇、异麦芽酮糖、D-山梨醇及D-甘露醇,同时阐释了9种甜味剂的二级质谱裂解途径。结论:方法简单快速,每次检测时间仅需几秒,检测效率比液质联用法提高近25倍,适用于大批量葡萄酒中甜味剂类成分的快速筛查。

Objective: To develop a rapid analysis method of 9 sweeteners:acesulfame K,aspartame,neotame,glycyrrhetic acid,lactitol,palatinose,xylitol,D-sorbitol and D-mannitol in wines. Methods: Triple quadrupol MS with DART ion source was used to perform the direct analysis.The source voltage of DART was 350 V.The temperature was 350 ℃.The DART sampler delivery rate was 2 mm·s^-1.Daughter scan mode was directly used to analyze the samples.The identification was performed by comparing the mass spectra of detected peaks in samples with the mass spectra of peaks in reference substance. Results: The limits of detection for 9 sweeteners ranged from 2.5 μg·mL^-1 to 20 μg·mL^-1.Lactitol,palatinose,D-sorbitol and D-mannitol were detected in 5 samples.Additionally,the fragmentation pathways of the 9 sweeteners were speculated. Conclusion: The method is easy and accurate.The time of once detection is only several seconds.The efficiency of detection is improved by about 25 times compared with HPLC-MS.The method can be used to rapidly detect and identify the 9 sweeteners in abundant batch of wines.