HPLC-ELSD及GC-MS法分析参麦注射液及中间体糖类成分

目的: 对参麦注射液及其中间体的糖类化合物进行定性分析。方法: 首先采用HPLC-ELSD结合Alltech Prevail^TM Carbohydrate ES色谱柱(250 mm×4.6 mm,4 μm)对糖类型做初步判断,流动相为乙腈-水(70:30),再利用Agilent气相色谱-质谱联用仪结合Agilent HP-5MS玻璃毛细管色谱柱(30 m×0.25 mm,膜厚0.25 μm)对含有的糖类化合物进行分析。结果: HPLC-ELSD法可以鉴定出参麦注射液及其中间体仅含三糖以下的糖。GC-MS法进一步分析显示麦冬醇提液主要含葡萄糖、果糖、蔗糖3种糖,红参醇提液和参麦注射液中主要含葡萄糖、果糖、蔗糖、麦芽糖4种糖。结论: 本法可以快速鉴定出参麦注射液及其中间体样品的糖类化学成分。

Objective: To conduct qualitative analysis of saccharides in Shenmai injection and its intermediates. Methods: Initial judgment was performed on the type of saccharides using HPLC-ELSD combined with Alltech Prevail^TM Carbohydrate ES(250 mm×4.6 mm,4 μm)column with acetonitrile-water(70:30)as the mobile phase;and then saccharides were analyzed using Agilent GC-MS combined with Agilent HP-5MS(30 m×0.25 mm,0.25 μm)column. Results: The samples that were composed of saccharides which were less than trisaccharide can be accurately identified using HPLC-ELSD.The further analysis using GC-MS showed that the Ophiopogonis Radix intermediate contained glucose,fructose and sucrose,and the Ginseng Radix et Rhizoma Rubra intermediate and the Shenmai injection mainly contained 4 kinds of sugar compositions,i.e.,glucose,fructose,sucrose,and maltose. Conclusion: This method can quickly identify the carbohydrate chemical composition in Shenmai injection and its intermediate samples.