高效液相色谱法同时测定栀子金花丸中9个成分的含量

目的：建立高效液相色谱法同时测定栀子金花丸中9个成分的方法。 方法：采用DIKMA Platisil ODS色谱柱(250 mm×4.6 mm，5 μm)， 柱温35 ℃，流动相为甲醇-0.1%磷酸水溶液，梯度洗脱(0~5 min，40%A；5~20 min，40%A→60%A；20~26 min，60%A→60%A；26~35 min，60%A→90%A；35~52 min，90%A；52~54 min，90%A→40%A)， 流速为1 mL·min^-1，变换波长检测(327 nm，绿原酸、盐酸小檗碱；254 nm，栀子苷、黄芩苷、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚)。 结果：绿原酸、栀子苷、盐酸小檗碱、黄芩苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的线性范围分别为0.02~0.4 μg(r=0.9998)、0.049~0.98 μg(r=1.000)、0.05~1 μg(r=0.9999)、0.1~2 μg(r=1.000)、0.0025~0.05 μg(r=0.9996)、0.001~0.02 μg(r=0.9997)、0.0014~0.028 μg(r=0.9998)、0.004~0.08 μg(r=0.9998)、0.00033~ 0.0066 μg(r=0.9998)，平均回收率在98.8%~100.4%之间。 结论：该法经方法学验证，是可用于栀子金花丸质量控制的一个有效的方法。

Objective: To establish a method for simultaneous determination of chlorogenic acid,geniposide,berberine,baicalin,aloe-emodin,rhein,emodin,chrysophanol and physcion in Zhizi Jinhua pills. Methods: The separation was performed on a DIKMA Platisil ODS column(250 mm×4.6 mm,5 μm)with gradient elution(0-5 min,40%A;5-20 min,40%A→60%A;20-26 min,60%A→60%A;26-35 min,60%A→90%A;35-52 min,90%A;52-54 min,90%A→40%A)using methanol and 0.1% phosphoric acid aqueous solution as the mobile phase.The flow rate was 1 mL·min^-1 and the detection wavelengths were 327 nm for chlorogenic acid and berberine,254 nm for geniposide,baicalin,aloe-emodin,rhein,emodin,chrysophanol and physcion,respectively. Results: Linear ranges of chlorogenic acid,geniposide,berberine,baicalin,aloe-emodin,rhein,emodin,chrysophanol and physcion concentrations were 0.02-0.4 μg(r=0.9998),0.049-0.98 μg(r=1.000),0.05-1 μg(r=0.9999),0.1-2 μg(r=1.000),0.0025-0.05 μg(r=0.9996),0.001-0.02 μg(r=0.9997),0.0014-0.028 μg(r=0.9998),0.004-0.08 μg(r=0.9998),0.00033-0.0066 μg(r=0.9998),respectively.Their average recoveries fell between 98.8% and 100.4%. Conclusion: The methodology validation shows that this HPLC method established can be used to determine the contents of chlorogenic acid,geniposide,berberine,baicalin,aloe-emodin,rhein,emodin,chrysophanol and physcion in Zhizi Jinhua pills.The method is an effective method for the quality control of Zhizi Jinhua pills.