疏血通、血塞通、血栓通注射液的抗凝血酶活性测定及比较

目的：定量测定疏血通注射液、血塞通注射液、血栓通注射液的抗凝血酶活性。 方法：建立了紫外可见分光光度法与抗凝法相结合的方法，测定了凝血酶时间与凝血酶效价的常用对数的标准曲线，其中线性方程Y=-0.8954X+2.190，r=-0.9999，线性范围为0.232~1.16 U·mL^-1。 结果：疏血通注射液、血塞通注射液、血栓通注射液均具有抗凝血酶活性，但三者的抗凝血酶活性有显著性差异。疏血通注射液的抗凝血酶活性为10.6~13.0 U·mL^-1；血塞通注射液的抗凝血酶活性为6.9~13.6 U·mL^-1；血栓通注射液的抗凝血活性为4.6~9.8 U·mL^-1。 结论：疏血通注射液和血塞通注射液的抗凝血酶活性高于血栓通注射液。

Objective: To establish a method for the detection and comparison of anti-thrombin activity of Shuxuetong,Xuesaitong and Xueshuantong injection. Methods: A method was created for detecting the anti-thrombin activity with the combination of the UV-vis spectrophotometer and the anticoagulation method.The standard curve of the logarithm of thrombin time and titer was measured.The linear equation was Y=0.8954X+2.190(r=-0.9999)and the linear range was 0.232-1.16 U mL^-1. Results: Shuxuetong,Xuesaitong and Xueshuantong injection all had anti-thrombin activity.However,a significant difference was found among them.The anti-thrombin activities of Shuxuetong,Xuesaitong and Xueshuangtong were 10.6-13.0,6.9-13.6 and 4.6-9.8 U·mL^-1,respectively. Conclusion :The anti-thrombin effect of Shuxuetong injection and Xuesaitong injection is higher than that of Xueshuantong injection.

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