HPLC法同时测定小儿柴桂退热颗粒中葛根素、大豆苷、黄芩苷、大豆苷元、桂皮醛、黄芩素的含量

目的： HPLC法同时测定小儿柴桂退热颗粒中葛根素、大豆苷、黄芩苷、大豆苷元、桂皮醛、黄芩素的含量。 方法： 采用Agilent ZORBAX SB-C₁₈色谱柱（250 mm×4.6 mm，5 μm），以乙腈（A）-0.1%磷酸水溶液（B）为流动相梯度洗脱（0~5 min，93%B；5~20 min，93%B→89%B；20~23 min，89%B→85%B；23~30 min，85%B→83%B；30~ 35 min，83%B→77%B；35~50 min，77%B→52%B；50~53 min，52%B→15%B；53~55 min，15%B），流速1.0 mL·min^-1，检测波长250 nm（0~35 min，测定葛根素和大豆苷）、270 nm（35~48 min，测定黄芩苷和大豆苷元）、290 nm（48~50.5 min，测定桂皮醛）、278 nm（50.5~55 min，测定黄芩素），柱温40 ℃。 结果： 葛根素、大豆苷、黄芩苷、大豆苷元、桂皮醛、黄芩素进样量分别在0.01240~2.480、0.004208~ 0.8416、0.005503~1.101，0.002132~0.4264、0.0007120~0.1424、0.0007980~0.1597 μg范围内与峰面积均有较好的线性关系，平均加样回收率均在99.0%~101.2%范围内，RSD均小于1.3%。 结论： 经方法学验证，本法可用于小儿柴桂退热颗粒中葛根素、大豆苷、黄芩苷、大豆苷元、桂皮醛、黄芩素的含量测定，为小儿柴桂退热颗粒全面的质量控制提供科学依据。

Objective: To simultaneously determine puerarin,d aidzin,baicalin,daidzein,cinnamaldehyde and baicalein in Xiaoer Chaigui Tuire Keli by HPLC. Methods: HPLC experiments were performed on Agilent ZORBAX SB-C₁₈(250 mm×4.6 mm,5 μm),eluted with acetonitrile (A) - 0.1% phosphoric acid (B) as the mobile phases in gradient mode(0-5 min,93%B;5-20 min,93%B→89%B;20-23 min,89%B→85%B;23-30 min,85%B→83%B;30-35 min,83%B→77%B;35-50 min,77%B→52%B;50-53 min,52%B→15%B;53- 55 min,15%B).The detection wavelength was set as follows:0-35 min,250 nm for puerarin and daidzin;35-48 min,270 nm for baicalin and daidzein;48-50.5 min,290 nm for cinnamaldehyde;50.5-55 min,278 nm for baicalein.The column temperature was at 40 ℃. Results: The good linearity with peak areas was achieved in the range of 0.01240-2.480 μg for puerarin,0.004208-0.8416 μg for daidzin,0.005503-1.101 μg for baicalin,0.002132-0.4264 μg for daidzein,0.000712-0.1424 μg for cinnamaldehyde,0.000798-0.1597 μg for baicalein.The average recoveries were between 99.04% and 101.2% with RSD less than 1.3%. Conclusion: The method can be used in determination of Xiaoer Chaigui Tuire Keli,puerarin,daidzin,baicalin,daidzein,cinnamaldehyde,baicalein for the comprehensive quality control by method validation,which can be used for the overall assessment of the quality of Xiaoer Chaigui Tuire Keli.