UPLC法同时测定三七伤药片中4个成分含量

目的：建立超高效液相色谱法同时测定三七伤药片中芍药苷、柚皮苷、人参皂苷Rg₁及人参皂苷Rb₁ 4个成分的含量。方法：采用BEH C₁₈色谱柱（50 mm×2.1 mm，1.7 μm），以乙腈-水为流动相，梯度洗脱，流速0.4 mL·min^-1，检测波长230 nm（0~5 min，芍药苷）、283 nm（5~10 min，柚皮苷）、203 nm（10~30 min，人参皂苷Rg₁及人参皂苷Rb₁），柱温40 ℃，进样量1 μL。结果：芍药苷、柚皮苷、人参皂苷Rg₁、人参皂苷Rb₁浓度在相应范围内与峰面积呈现良好的线性关系。不同厂家的三七伤药片4个成分的含量参差不齐，君药三七中人参皂苷Rg₁及人参皂苷Rb₁的含量差距尤为显著。结论：该法可同时测定三七伤药片中4个成分的含量，分析速度快，溶剂用量少，重复性好，为三七伤药片的质量评价提供参考。

Objective: To establish a UPLC method to simultaneously determine 4 kinds of constituents in Sanqi Shangyao tablets, namely,paeoniflorin,naringin,ginsenosides Rg₁ and Rb₁. Methods: The BEH C₁₈ column(50 mm ×2.1 mm,1.7 μm)was used with the mobile phase of acetonitrile-water gradient elution system at a velocity of 0.4 mL·min^-1;the wavelength was 230 nm(0-5 min)for paeoniflorin,283 nm(5-10 min)for naringin,203 nm(10-30 min)for ginsenosides Rg₁ and Rb₁.The column temperature was 40 ℃;the injection volume was 1 μL. Results: Paeoniflorin,naringin,ginsenosides Rg₁ and Rb₁ showed good linear relationship respectively with the peak area in the corresponding range of concentration.The content of four constituents of Sanqi Shangyao tablets varied a lot among different manufactures,and the difference between ginsenosides Rg₁ and Rb₁ in monarch drug of Radix Notoginseng was especially obvious. Conclusion: The UPLC method can be used for the simultaneous determination of 4 constituents in Sanqi Shangyao tablets with the fast analysis speed,small amount of solvent and good repeatability,which provides a reference method for quality assessment of Sanqi Shangyao tablets.