市售化橘红质量评价

目的： 采用HPLC法测定化橘红药材中柚皮苷、柚皮芸香苷、野漆树苷的含量，考察市售化橘红药材的质量现状。 方法： 采用Kromasil C₁₈（5 μm， 250 mm×4.6 mm）柱，以甲醇-0.1磷酸（47：53）为流动相，流速为1.0 mL·min^-1，检测波长为283 nm。 结果： 柚皮苷、柚皮芸香苷、野漆树苷进样量分别在0.061～3.05 μg、0.041～2.05 μg、0.040～2.01 μg范围内呈良好线性关系；平均加样回收率（n=5）分别为97.5%（RSD=1.2%）、97.2%（RSD=1.4%）和96.2%（RSD=1.1%）。 结论： 本文试验收集的43份样品测定结果显示化橘红中以上3种成分的含量差异较大。

Objective: To study the quality of Exocarpium Citri Grandis by determining the contents of naringin,narirutin,and rhoifolin. Methods: The three flavonoids were separated by HPLC on C₁₈ column(5 μm, 250 mm×4.6 mm)with methanol-0.1% phosphoric acid water(47:53)as the mobile phase and detected at 283 nm. Results: The linear range of naringin,narirutin and rhoifolin was 0.061-3.05 μg,0.041-2.05 μg,and 0.040-2.01 μg,respectively;the average recovery (n=5)was 97.5%(RSD=1.2%), 97.2%(RSD=1.4%) and 96.2%(RSD=1.1%), respectively. Conclusion: There was significant difference in the content of 3 components of Exocarpium Citri Grandis sold on market.

[1] LIN Li(林励),OU Jian-feng(欧剑锋),LIAO Guan-rong(廖观荣),et al.Studies on genuineness of Exocarpium Citri Grandis(化橘红道地性的初步研究)[J].J Guangzhou Univ Tradit Chin Med(广州中医药大学学报),2010,27(3):163
[2] LIU Xiao-han(刘晓涵),CHEN Yong-gang(陈永刚),LIN li(林励),et al.Simultaneous determination of naringin and rhoifolin in Exocarpium Citri Grandis by RP-HPLC(化橘红中柚皮苷和野漆树苷含量同时测定方法的建立)[J].Tradit Chin Drug Res Clin Pharmacol(中药新药与临床药理),2010,21(6):640
[3] ChP 2010.Vol Ⅰ(中国药典2010年版.一部).2010:69
[4] LIN Li(林励),CHEN Zhi-xia(陈志霞),YUAN Xu-jiang(袁旭江),et al.Assessment of the quality of two different species of Exocarpium Citri Grandis(两种化橘红的质量鉴别)[J].J Guangzhou Univ Tradit Chin Med(广州中医药大学学报),2004,21(7):308
[5] CHEN Zhi-xia(陈志霞),LIN Li (林励),SUN Dong-mei(孙冬梅).HPLC fingerprint of flavonoids in Exocarpium Citri Grandis(化橘红黄酮类成分的HPLC指纹图谱研究)[J].Chin Tradit Herbal Drugs(中草药),2003,7(7):657
[6] WEI Min(韦敏),YANG Zhong-lin(杨中林),WU Jian-min(吴建民).Separation and purification of naringin from Citrus grandis Tomentosa by macroporous adsorption resin(大孔吸附树脂法分离纯化化橘红中柚皮苷的研究)[J].Chin Tradit Pat Med(中成药),2007,29(3):352
[7] CHEN Jian-hua(陈建华),LIN Huan-ze(林焕泽),LIAO Jing-hui(廖景辉).Study on pharmacognosy of Citrus grandis 'tomentosa' and Citrus grandis(L.)osbeck of Exocarpium citri grandis' source(化橘红生源化州柚与柚的生药学研究)[J].China Mod Med(中国当代医药),2011,18(35):56
[8] DENG Shao-dong(邓少东),WANG Lian-jing(王莲婧),LIN Li(林励).UPLC determination of four flavonoids in exocarpium Citrus grandis before and after acid hydrolysis(UPLC法测定酸水解前后化橘红中4种黄酮类成分的含量)[J].Chin J Tradit Chin Med(中华中医药杂志),2012,27(4):928
[9] ZHANG Chao-hong(张超洪),XIAO Wei-qiang(肖维强),ZHAO Peng(赵鹏).Determination of flavanones in tomentose pummelo from Guangdong Province by high-performance liquid chromatography(HPLC)(广东省化橘红中黄酮类物质的HPLC测定)[J].J Huazhong Agric Univ(华中农业大学学报),2009,28(4):483
[10] LI Run-tang(李润唐),ZHANG Ying-nan (张映南),LI Ying-zhi(李映志 ).Research advance on Huajuhong(化橘红研究进展)[J].Guangdong Forest Sci Technol(广东林业科技),2008,24(2):82
[11] LIU Qun-di(刘群娣),XU Xin-jun(徐新军),WAN Jin-zhi(万金志).High-speed counter-current chromatography preparation and separation ofnaringin,meranzin hydrate and isomeranzin from Exocarpium Citri Grandis(高速逆流色谱法分离制备化橘红中的柚皮苷、橙皮内酯水合物和异橙皮内酯)[J].Chin J Pharm Anal(药物分析杂志),2011,31(5):831
[12] CHEN Jian-hua(陈建华),LIN Huan-ze(林焕泽),LIAO Jing-hui(廖景辉).Study on pharmacognosy of Citrus grandis 'tomentosa' and Citrus grandis(L.)osbeck of Exocarpium citri grandis' source(化橘红生源化州柚与柚的生药学研究)[J].China Mod Med(中国当代医药),2011,18(35):56