HPLC法同时测定栀子类药材中10个主要有效成分的含量

目的： 建立了同时测定22批栀子类药材中10个主要有效成分（京尼平苷酸、去乙酰基车叶草苷、羟基栀子苷、鸡矢藤次苷甲酯、京尼平龙胆双糖苷、京尼平苷、对香豆酰京尼平龙胆双糖苷、西红花苷-1、西红花苷-2、西红花苷-3）的高效液相色谱检测方法。 方法： 采用Agilent Zorbax SB-C₁₈ （4.6 mm×250 mm，5 μm） 色谱柱，以乙腈（A）-0.2%磷酸溶液（B）为流动相，梯度洗脱，流速 1.0 mL·min^-1；检测波长分别为238 nm、440 nm。 结果： 在优化的色谱条件下，10个有效成分浓度在2.16～905 μg·mL^-1范围内线性关系良好，相关系数（r）不低于0.9998，检出限为0.02～0.20 μg·mL^-1。22批栀子类药材的含量测定结果表明，京尼平苷是最主要的环烯醚萜类成分，西红花苷-1是最主要的西红花苷类成分。不同产地18批栀子药材中10个成分的含量差异较大，其中京尼平苷、西红花苷-1含量分别为2.30%～4.69%、0.20%～1.06%。 结论： 该方法为栀子类药材的质量控制提供科学依据。

Objective: To develop an HPLC method for determination of 10 compounds (geniposidic acid,deacetylasperulosidic acid methyl ester,gardenoside,scandoside methyl ester,genipin-1-β-D-gentiobioside,geniposide,p-trans-coumaroylgenipin gentiobioside,crocin-1,crocin-2,crocin-3) in fruits of Gardenia. Methods: The HPLC separation was performed on an Agilent Zorbax SB-C₁₈ (4.6 mm×250 mm,5 μm) column eluted with the mobile phases of acetonitrile-0.2 % phosphoric acid solution in a gradient mode at a flow rate of 1.0 mL·min^-1.The detection wavelength was 238 nm and 440 nm. Results: The chromatographic peaks of ten compounds and the corresponding spectra were ideal under the optimal conditions.The calibration curves of ten analytes were linear in the range of 2.16-905 μg·mL^-1,with correlation coefficients more than 0.9998.The limits of detection (LODs) of ten analytes were in the range of 0.02-0.20 μg·mL^-1.As the results of determination of 10 compounds of 22 batches of Gardenia showed,geniposide was the main iridoid substance;crocin-1 was the most important crocin substance.There was a great difference in the contents of 10 compounds in 18 batches of Fructus Gardeniae,the contents of geniposide were 2.30%-4.69%,the contents of crocin-1 were 0.20%-1.06%. Conclusion: The method can provide reference for evaluating the quality of fruits of Gardenia.