HPLC切换波长法同时测定白蔹中没食子酸、原儿茶醛、儿茶素和白藜芦醇的含量

目的：采用反相高效液相色谱切换波长法建立同时测定白蔹中没食子酸、原儿茶醛、儿茶素、白藜芦醇4个成分含量的方法。方法：采用 Agilent Zorbax Eclipse plus 色谱柱（4.6 mm×250 mm，5 μm）；流动相为乙腈-0.1%磷酸水溶液，梯度洗脱；流速：1.0 mL·min^-1；检测波长：270 nm（0~27 min），360 nm（27.01~36 min），306 nm（36.01~40 min）；柱温：30 ℃。结果：没食子酸、原儿茶醛、儿茶素、白藜芦醇的进样量分别在0.0522~1.044 μg（r=0.9993）、0.0548~1.096 μg（r=0.9992）、0.4004~8.008 μg（r=0.9990）和0.0086~0.172 μg（r=0.9993）范围内，与色谱峰峰面积呈良好线性响应；平均回收率分别为100.5%、98.16%、99.60%、99.80%（n=6）。结论：该方法经方法学验证可用于白蔹的质量控制。

Objective: To establish a wavelength switching method for simultaneous determination of gallic acid,protocatechuic aldehyde,catechin,and resveratrol in Ampelopsis japonica root. Methods: The Agilent Zorbax Eclipse plus chromatographic column (4.6 mm×250 mm,5 μm) was adopted.The mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) with gradient elution at a flow rate of 1.0 mL·min^-1.The detection wavelength was 270 nm (0-27 min,for gallic acid,protocatechuic aldehyde,and catechin),360 nm (27.1-36 min) and 306 nm (36.01-40 min,for resveratrol).The column temperature was 30 ℃. Results: The method achieved a good linearity in the ranges of 0.0522-1.044 μg (r=0.9993) for gallic acid,0.0548 -1.096 μg (r=0.9992) for protocatechuic aldehyde,0.4004 -8.008 μg (r=0.9990) for catechin,and 0.0086-0.172 μg(r=0.9993) for resveratrol.The average recoveries of gallic acid,protocatechuic aldehyde,catechin and resveratrol were 100.5%,98.16%,99.60%,99.80% (n=6),respectively. Conclusion: This method can be used to control the quality of Ampelopsis japonica root by methodology validation.