HPLC法测定柘木中槲皮素、染料木素和山柰素的研究

目的：采用高效液相色谱法同时测定柘木中3个黄酮类成分（槲皮素、染料木素和山柰素）的含量。方法：采用Apollo C₁₈色谱柱（4.6 mm×250 mm，5 μm），以1%醋酸-甲醇为流动相，梯度洗脱（0~28 min，65%A→40%A）；流速：1 mL·min^-1；柱温：30 ℃；检测波长：260 nm。结果：槲皮素、染料木素和山柰素进样量分别在0.159~1.59 μg（r=0.9997）、0.046~0.46 μg（r=0.9995）和0.048~0.48 μg（r=0.9998）范围内线性关系良好；平均回收率（n=6）分别为98.2%（RSD=2.6%）、100.6%（RSD=2.9%）和100.4%（RSD=2.7%）。结论：本法为该药材质量控制研究提供了参考。

Objective: To develop a method for determining 3 flavonoid constituents (quercetin,genistein and kaempferol) in Cudrania tricuspidata. Methods: The analysis was conducted on an Apollo C₁₈ column (4.6 mm×250 mm,5 μm) eluted with 1% acetic acid and methanol as mobile phases in gradient mode (0-28 min,65%A→40%A). The flow rate was 1 mL·min^-1 and the detection wavelength was set at 260 nm. The column temperature was 30 ℃. Results: The linear concentration ranges of quercetin,genistein and kaempferol were 0.159-1.59 μg (r=0.9997),0.046-0.46 μg (r=0.9995) and 0.048-0.48 μg (r=0.9998),respectively;The average recoveries (n=6) were 98.2% (RSD=2.6%),100.6% (RSD=2.9%) and 100.4% (RSD=2.7%),respectively. Conclusion: The method is simple,accurate and sensitive,and can be used for the quality control of Cudrania tricuspidata as a reference.