RP-HPLC同时测定公丁香中5个三萜酸的含量

目的：采用超声辅助提取，建立反相高效液相色谱法同时测定公丁香中山楂酸、科罗索酸、白桦脂酸、齐墩果酸和熊果酸的含量。方法：色谱柱为Kromasil C₁₈柱（4.6 mm×250 mm，5 μm），流动相为甲醇-水-磷酸（86：14：0.1），流速1.0 mL·min^-1，检测波长为210 nm，柱温为30 ℃。结果：山楂酸、科罗索酸、白桦脂酸、齐墩果酸和熊果酸线性范围分别为0.0488~1.220 μg（r=0.9994）、0.0856~2.140 μg（r=0.9996）、0.1680~4.200 μg（r=0.9999）、0.2024~5.060 μg（r=0.9997）和0.2224~5.560 μg（r=0.9999），平均回收率（n=6）分别为100.9%、98.94%、98.25%、99.55%、101.1%，RSD分别为2.0%、2.0%、1.5%、0.39%、1.4%。结论：该方法经方法学验证可用于公丁香样品中三萜酸的含量测定。

Objective: To establish a RP-HPLC method for simultaneous determination of maslinic acid,corosolic acid,betulinic acid,oleanolic acid and ursolic acid in the flower buds of Eugenia caryophyllata by ultrasonic wave aided method. Methods: The separation was achieved on a Kromasil C₁₈ column (4.6 mm×250 mm,5 μm) at 30 ℃ using methanol-water-phosphoric acid solution (86:14:0.1) as the mobile phase at a flow rate of 1.0 mL·min^-1 and 210 nm as the detection wavelength. Results: The linear ranges of maslinic acid,corosolic acid,betulinic acid,oleanolic acid,and ursolic acid were 0.0488-1.220 μg (r=0.9994),0.0856-2.140 μg (r=0.9996),0.1680-4.200 μg (r=0.9999),0.2024-5.060 μg (r=0.9997) and 0.2224-5.560 μg (r=0.9999),respectively. The average recoveries (n=6) were 100.9%,98.94%,98.25%,99.55%,and 101.1%,and RSDs were 2.0%,2.0%,1.5%,0.39%,and 1.4%,respectively. Conclusion: The methodology validation shows that The proposed method is accurate,simple and reliable,which is applicable for the determination of triterpenic acid in the flower buds of Eugenia caryophyllata.