桂枝茯苓丸脂溶性成分GC-MS指纹图谱研究

目的：建立桂枝茯苓丸脂溶性成分的气相色谱-质谱联用（GC-MS）指纹图谱。方法：采用5HP-MS色谱柱（30 m×0.25 mm×0.25 μm），载气为He，流速1.0 mL·min^-1，进样量1 μL，分流比1：20;采用电子轰击（EI）离子源，辅助线温度280℃，离子源温度230℃，四极杆温度150℃，质量扫描范围m/z 50～550。采用Chemstation和Nist 05a谱库。结果：分析了10个批次的桂枝茯苓丸，建立了桂枝茯苓丸脂溶性成分GC-MS的指纹图谱，确定并鉴定了9个共有色谱峰。采用中药色谱指纹图谱相似度评价系统软件（2004 A版）进行指纹图谱分析，10批桂枝茯苓丸相似度均在0.90以上。结论：桂枝茯苓丸脂溶性成分的GC-MS指纹图谱特征性及专属性强，该指纹图谱可作为桂枝茯苓丸脂溶性成分质量控制的稳定方法。

Objective: To establish GC-MS fingerprints of fat-soluble constituents for Guizhi Fuling pills. Methods: Chromatographic conditions were as follows: the 5HP-MS quartz capillary column(30 m×0.25 mm×0.25 μm) was adopted with the carrier gas of He at a velocity of 1.0 mL·min^-1,the injection volume was 1 μL,and the split ratio was 1: 20. MS conditions were as follows: the electron impact(EI) ion source was adopted;the transmission line temperature was at 280℃,the ion source temperature was at 230℃,the quadrupole temperature was at 150℃;the mass scan range was m/z 50-550. And the results were compared with the spectra library of Chemstation and Nist 05a. Results: Nine common peaks were found in the GC-MS fingerprint of fat-soluble constituents from Guizhi Fuling pills. The operating standard of similarity evaluation system for chromatographic fingerprints of Chinese Materia Medica (Version 2004 A) was used to calculate. Similarity of 10 batches of Guizhi Fuling pill samples was higher than 0.90. Conclusion: The fingerprinting can be used as a method of quality control and can provide evidence for the research of fat-soluble constituents of Guizhi Fuling pills.