在线渗析-离子色谱法检测中药材中二氧化硫残留量

目的：建立了离子色谱法电化学检测器测定中药材中的亚硫酸盐（以二氧化硫计）的残留量。方法：采用碱溶液提取中药材，提取液经在线渗析技术去除大分子杂质，离子色谱法测定亚硫酸根离子。色谱条件:保护柱Metrosep RP2 Guard、分析柱Metrosep A Supp 10-100，以100 mmol·L^-1氢氧化钠溶液作为淋洗液，流速为1.0 mL·min^-1，采用安培检测器检测。结果：亚硫酸钠浓度在2.042～51.05 μg·mL^-1范围内线性关系良好，r=0.9999。结论：本方法操作简便，干扰少，选择性高，测定结果较为准确，可用于中药材中二氧化硫残留量的研究。

Objective: To establish a method for the determination of sulfite residues(in terms of sulfur dioxide) in the Chinese materia medica by ion chromatography(IC). Methods: After extraction with alkali solution,the sample solutions were treated with the inline dialysis sample pretreatment system to remove the impurities,and were then separated on a Metrosep A Supp 10-100 column with 100 mmol·L^-1 sodium hydroxide solution as the eluting agent at the flow rate was 1.0 mL·min^-1.The detector was amperometric detector. Results: The linearity range was 2.042-51.05 μg·mL^-1,r=0.9999. Conclusion: This simple method has high selectivity and low interference for the determination of sulfur dioxide residues in Chinese materia medica.

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