高效液相色谱法测定调脂胶囊中3个皂苷的含量

目的: 建立高效液相色谱法测定调脂胶囊中三七皂苷R₁、人参皂苷Rg₁、人参皂苷Rb₁的含量。 方法: 采用Venusil C₁₈色谱柱(4.6 mm×250 mm,5 μm),以乙腈(A)-水(B)为流动相,四阶梯式梯度洗脱 81);35~80 min,A-B(29:71);80~105 min,A-B(40:60);105~120 min,A-B(19:81)],流速1.0 mL·min^-1,检测波长203 nm,柱温为室温。 结果: 三七皂苷R₁、人参皂苷Rg₁、人参皂苷Rb₁分别在2.21~13.28 mg、0.64~3.87 mg、2.80~16.82 mg范围内有良好的线性关系;平均回收率(n=9)分别为98.9%、97.4%、99.5%。 结论: 本方法结果准确,灵敏度高,重复性好,可作为调脂胶囊质量控制的方法。

Objective: To establish a RP-HPLC method for the determination of 3 saponins(notoginsenoside R₁,ginsenoside Rg₁,ginsenoside Rb₁)in Tiaozhi capsules. Methods: The analysis was carried out on a C₁₈ column(4.6 mm×250 mm,5 μm)with acetonitrile(A)-water(B)as the mobile phase by the four-step gradient elution 81);35-80 min,A-B(29: 71);80-105 min,A-B(40: 60);105-120 min,A-B(19: 81)],the flow rate was 1.0 mL·min^-1,the detection wavelength was 203 nm,and the column temperature was room temperature. Results: The linear ranges of notoginsenoside R₁,ginsenoside Rg₁ and ginsenoside Rb₁ were 2.21-13.28 mg,0.64-3.87 mg,2.80-16.82 mg,respectively.The average recoveries(n=9)of notoginsenoside R₁,ginsenoside Rg₁ and ginsenoside Rb₁ were 98.9%,97.4%,99.5%,respectively. Conclusion: This method is simple and accurate with high sensitivity and good repeatability,omd which can be used for quality control of Tiaozhi capsules.