目的: 建立HPLC法同时测定麻仁润肠丸中芍药苷、橙皮苷、芦荟大黄素、大黄酸、大黄素、木香烃内酯、去氢木香内酯、大黄酚、大黄素甲醚的含量。 方法: 色谱柱:Inertsil®ODS-SP(4.6 mm×250 mm,5 μm);流动相:0.1%磷酸溶液-乙腈梯度洗脱;流速:1.0 mL·min-1;检测波长:230 nm;柱温:35℃;进样量:10 μL。 结果: 在上述色谱条件下,麻仁润肠丸中9个成分之间有良好的分离度,芍药苷、橙皮苷、芦荟大黄素、大黄酸、大黄素、木香烃内酯、去氢木香内酯、大黄酚和大黄素甲醚的线性范围分别是2.045~81.79、4.063~162.5、0.2952~11.81、0.5184~20.74、0.4612~18.49、0.6120~24.48、0.9348~37.39、1.034~41.34、0.4912~19.65 μg·mL-1;r分别为0.9988、0.9943、0.9998、0.9999、0.9999、0.9995、0.9996、0.9939、0.9914;平均加样回收率(n=6)分别为98.3%、97.5%、98.5%、100.5%、101.7%、101.3%、97.1%、100.5%、100.0%,RSD分别为0.90%、0.89%、1.6%、1.0%、1.0%、1.6%、0.82%、1.2%、0.99%。 结论: 本法操作简单、可靠,重复性好,为更好地控制麻仁润肠丸的质量提供参考依据。
Objective: To establish an HPLC method for simultaneous determination of paeoniflorin,hesperidin,aloe-emodin,rhein,emodin,costunolide,dehydrocostus lactone,chrysophanol and physcion in Maren Runchang pills. Methods: The Inertsil®ODS-SP(4.6 mm×250 mm,5 μm)was used,the column temperature was maintained at 35℃.The UV detection wavelength was at 230 nm.A gradient elution of acetonitri-0.1% phosphoric acid was adopted at the flow rate of 1.0 mL·min-1.The injection volume was 10 μL. Results: The good resolution was achieved among the 9 active ingredients.The linear ranges of paeoniflorin,hesperidin,aloe-emodin,rhein,emodin,costunolide,dehydrocostus lactone,chrysophanol and physcion were 2.045-81.79 μg·mL-1(r=0.9988),4.063-162.5 μg·mL-1(r=0.9943),0.2952-11.81 μg·mL-1(r=0.9998),0.5184-20.74 μg·mL-1(r=0.9999),0.4612-18.49 μg·mL-1(r=0.9999),0.6120-24.48 μg·mL-1(r=0.9995),0.9348-37.39 μg·mL-1(r=0.9996),1.034-41.34 μg·mL-1(r=0.9939)and 0.4912-19.65 μg·mL-1(r=0.9914)respectively.The average recoveries(n=6)were 98.3%,97.5%,98.5%,100.5%,101.7%,101.3%,97.1%,100.5%,and 100.0%,with RSDs of 0.90%,0.89%,1.6%,1.0%,1.0%,1.6%,0.82%,1.2%,and 0.99%,respectively. Conclusion: The method is accurate,easy and reproducible,which can be applied to quality control of Maren Runchang pills.
