HPLC-MS/MS法同时测定新疆一枝蒿药材中5个成分的含量

目的: 建立新疆一枝蒿药材中绿原酸、木犀草素、一枝蒿酮酸、紫花牡荆素及芹菜素5个指标成分的含量测定方法, 比较不同产地及不同采收期新疆一 枝蒿药材中5个成分的含量。 方法: 采用HPLC-MS/MS法,使用Agilent C₁₈色谱柱(4.6 mm×250 mm,5 μm), 流动相为甲醇-0.2%甲酸溶液,梯度洗脱,流速1.0 mL·min^-1,柱温30℃,进样量10 μL;质谱采用电喷雾离子源(ESI源),负离子模式检测,扫描方式为多反应监测(MRM)。 结果: 绿原酸、木犀草素、一枝蒿酮酸、紫花牡荆素及芹菜素分别在52.4~1048 μg·mL^-1(r=0.9996)、2.965~118.6 μg·mL^-1(r=0.9997)、25~700 μg·mL^-1(r=0.9993)、5.96~238.4 μg·mL^-1(r=0.9994)、1.219~243.8 μg·mL^-1(r=0.9992)范围内线性关系良好;平均加样回收率在97.6%~100.9%之间,RSD均小于2.5%。 结论: 该方法灵敏、快速、准确,专属性强,可为新疆一枝蒿药材的质量标准研究提供依据。

Objective: To establish a method for simultaneous determination of 5 main bioactive constituents including chlorogenic acid,luteolin,rupestonic acid,casticin and apigenin in Artemisia rupestris L.by HPLC-MS/MS,and to compare the content differences of Artemisia rupestris L.from different areas and different harvest periods. Methods: The HPLC separation was performed on an Agilent C_l8 column(250 mm×4.6 mm,5 μm)in a gradient elute mode with methanol and water(containing 0.2% formic acid)as the mobile phase at a flow rate of 1.0 mL·min^-1.MS detection was performed with multiple reaction monitoring(MRM)mode using negative electrospray ionization. Results: The linearity was achieved in the range of 52.4-1048 μg·mL^-1(r=0.9996)for chlorogenic acid,2.965-118.6 μg·mL^-1(r=0.9997)for luteolin,25-700 μg·mL^-1(r=0.9993)for rupestonic acid,5.96-238.4 μg·mL^-1(r=0.9994)for casticin,and 1.219-243.8 μg·mL^-1(r=0.9992)for apigenin.The recoveries were 97.6%-100.9% with RSD less than 2.5%. Conclusion: The method is sensitive,rapid,accurate and specific and can be used for the quality control of Artemisia rupestris L.