近红外光谱法在秦艽提取过程中的应用

目的: 运用近红外光谱(NIR)分析技术建立龙胆苦苷定量分析模型,对秦艽提取过程中龙胆苦苷含量进行快速检测,实现在线监控。 方法: 收集秦艽中龙胆苦苷样本的近红外图谱,并用HPLC法测定其含量,根据含量值分为4个浓度段分别 建立近红外定量模型,并将所建模型应用于秦艽提取过程中在线检测龙胆苦苷含量。 结果: 定量模型的浓度范围为0.0024~63.3318 mg·mL^-1,所建模型的预测值与HPLC值偏差较小。在线应用时,龙胆苦苷含量的NIR值与HPLC值的浓度图谱趋势几乎一致。 结论: 建立的定量模型预测效果良好,能够在线控制秦艽提取过程中龙胆苦苷的含量。

Objective: To develop a near-infrared spectroscopy(NIR)quantitative analysis method for the rapid analysis and on-line determination of gentiopicroside in Gentiana. Methods: The near-infrared spectra of gentiopicroside were collected and the concentration value was determined by HPLC.Models of near-infrared spectra quantification were developed based on four different concentration ranges,and applied in the process of Gentiana extraction. Results: For the determination,the content range was 0.0024-63.3318 mg·mL^-1 and the model predictions had smaller deviation than HPLC determination value. Conclusion: The quantitative method has a well-predicted result,which can be applied in the process of Gentiana extraction on-line.

1 LU Qi-qin(芦启琴),LOU Deng-ji(娄灯吉).Chemical constituents and pharmacological activities of Radix Gentianae Macrophyllae(秦艽化学成分及药理作用研究进展).J Anhui Agric Sci(安徽农业科学),2007,35(29): 9299
2 NIE Li-xing(聂黎行),WANG Gang-li(王刚力),LI Zhi-meng(李志猛),et al.Qualitative and quantitative analysis of Tongren Wujibaifeng pills by near infrared spectroscopy(近红外光谱法对同仁乌鸡白凤丸的定性和定量分析).J Infrared Millim Waves(红外与毫米波学报),2008,27(3): 205
3 YAN Yan-lu(严衍禄),ZHAO Long-lian(赵龙莲),HAN Dong- hai(韩东海),et al.Near-infrared Spectroscopy Analytical Knowledge and Application(近红外光谱分析基础与应用).Beijing(北京): China Light Industry Press(中国轻工业出版社),2005.444
4 ZHANG Xing-wang(张兴旺),NIU Ying-feng(牛迎风),TAO Yan-duo(陶燕铎),et al.Study on the extraction process of gentiopicroside in Gentiana(秦艽中龙胆苦苷提取工艺研究).J Chin Med Mater(中药材),2009,32(4): 625
5 ZHAO Yong(赵勇),ZHANG Ru(张儒),SUN Wen-ji(孙文基).Study on the macroporous resin column enriched gentiopicroside in Gentiana(大孔吸附树脂富集秦艽叶中龙胆苦苷的工艺研究).J Chin Med Mater (中药材),2007,30(12): 1583
6 ChP(中国药典).2010.Vol Ⅰ(一部): 253
7 LIU Yan-yun(柳艳云),HU Chang-qin(胡昌勤),HANG Tai-jun(杭太俊).Study on the near-infrared spectral selection rules and elimination of the solvent interference of Acanthopanax injection(刺五加注射液近红外含量预测模型谱段选择规律和消除溶剂干扰方法的探讨).Sci China(Chim),2010,40(11): 1664
8 LU Wan-zhen(陆婉珍),YUAN Hong-fu(袁洪福),XU Guang- tong(徐广通),et al.Modern Near-infrared Spectroscopy Analytical Technology(现代近红外光谱分析技术).Beijing(北京): China Petrochemical Press(中国石化出版社),2007.165
9 Honigs DE,Hieftje GM,Hirschfeld T.An optimization of detector-noise-limited infrared multiwavelength determinations.Appl Spectrosc,1985,39(2): 253
10 QU Ling-bo(屈凌波),CHEN Guo-guang(陈国广),SHENG Long-sheng(盛龙生),et al.Near-infrared spectroscopy technology and its application in the analysis of drugs(近红外光谱技术及其在药物分析中的应用).Chin J Pharm Anal(药物分析杂志),1996,16(3): 204
11 HU Chang-qin(胡昌勤),FENG Yan-chun(冯艳春).Near-infrared Spectroscopy Technology on Drug Rapid Analysis(近红外光谱法快速分析药品).Beijing(北京): Chemistry Industry Press(化学工业出版社),2009.151
12 QIN Bing-da(覃炳达),LI Ling-qiao(李灵巧),YANG Hui-hua(杨辉华),et al.Application of OPC technology in near-infrared online intelligent monitoring system in production process of traditional Chinese medicine(OPC技术在中药生产过程近红外智能监控系统中的应用).Mod Comput(现代计算机),2011(7): 68