多波长HPLC法同时测定四神丸中7个有效成分的含量

目的: 建立多波长高效液相色谱法,同时测定四神丸中五味子醇甲、五味子甲素、五味子乙素、补骨脂素、异补骨脂素、去氢二异丁香酚、吴茱萸次碱7个有效成分的含量。 方法: 采用Diamond C₁₈色谱柱(4.6 mm×250 mm, 5 μm),柱温30 ℃,以乙腈-0.1%磷酸水溶液为流动相梯度洗脱(0 min,30%A;10 min,40%A;30 min,50%A;60 min,90%A;61~70 min,30%A),检测波长为220 nm (五味子醇甲、五味子甲素、五味子乙素)、280 nm(补骨脂素、异补骨脂素、去氢二异丁香酚)和345 nm(吴茱萸次碱)。 结果: 7个有效成分达到完全分离;线性关系良好;加样回收率在94.8%~104.1%。 结论: 建立的方法准确可靠,可用于四神丸的质量控制。

Objective: To develop an HPLC method with multiple wavelength ultraviolet detection for the simultaneous determination of seven active components(schisandrin, deoxyschizandrin, γ-schisandrin, psoralen, isopsoralen, dehydrodiisoeugenol, rutaecarpine) in Sishen pills. Methods: The samples were separated on a Diamond C₁₈ column(4.6 mm×250 mm, 5 μm) by gradient elution(0 min,30%A;10 min,40%A;30 min,50%A;60 min,90%A;61-70 min,30%A) using acetonitrile-0.1% phosphoric acid solution. The eluate was detected at multiple wavelengths of 220 nm for schisandrin, deoxyschizandrin and γ-schisandrin, 280 nm for psoralen, isopsoralen, dehydrodiisoeugenol and 345 nm for rutaecarpine. Results: The complete separation was obtained for the seven active compounds. The seven regression equations showed good linear relationships. The average recoveries of the compounds above were between 94.8% and 104.1%. Conclusion: The method is feasible, accurate and stable, which can control the quality of Sishen pills.

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