微波消解-ICP/MS法测定替曲朵辛中的13种微量金属元素

目的:建立替曲朵辛中金属元素的测定方法。方法: 采用微波消解-ICP/MS法测定替曲朵辛中的Al、Mn、 Ni、As、Pb、V、Fe、Cu、Cd、Cr、Co、Zn 和Ba 共13种金属元素。结果: 方法检出限为0.03~1.5×10^-9 g·g^-1,牛肝标准物质加标回收率在85%~109%,各元素线性关系良好。结论: 微波消解-ICP/MS法用量少、检出限低、分析速度快、能同时分析替曲朵辛中多种元素。

Objective: To establish a method for determination of heavy metal elements in tetrodotoxin. Methods: ICP/MS was used to determine 13 metal elements in tetrodotoxin,including Al,Mn, Ni,As,Pb,V,Fe,Cu,Cd,Cr,Co,Zn and Ba. Results: The method detection limits for all target elements were with a range of 0.03-1.5×10^-9 g·g^-1,while the recovery values ranged from 85% to 109%.The correlation coefficients were good. Conclusion: The microwave digestion-ICP/MS method has a low detection limit,a fast analysis rate and it only requires a small amount of sample which can simultaneously analyze various elements in tetrodotoxin.

1 Asakawa M,Toyoshima T,Shida Y,et al.Paralytic toxins in a ribbon worm Cephalothrix species(Nemertean)adherent to cultured oysters in Hiroshima Bay,Hiroshima Prefecture,Japan.Toxicon,2000,38(6):763
2 Yotsu M.Natural homologs of tetrodotoxin.What kind of metabolic differences exist between globefish and salamander discovery of 6-ep i-11-deoxy derivatives and a key for the elucidation of the biogenesis and metabolism of tetrodotoxin.Kagaku Seibutsu,1988,26(10):624
3 XU Ying(徐英),ZHANG Yong-he(张永鹤),KU Bao-shan(库宝善).Influence of tetrodotoxin on sodium channel and the possible mechanism of the analgesic effect(河豚毒素对钠通道的影响及其可能的镇痛机制). Chin Pharmacol Bull(中国药理学通报),2003,19(3):249
4 ZHANG Feng-yun(张凤云),KU Bao-shan(库宝善),YAO Hai-yan(姚海燕),et al.Analgesic and anti-inflammatory effects of tetrodotoxin alone and tetrodotoxin combined with indoxacarb(河豚毒素单用及与Indoxacarb联合应用的镇痛抗炎作用).Chin J Clin Rehabil(中国临床康复),2006,10(34):115
5 Daniel M,Schwaetz Z.Experimental study of tetrodotoxin:A long-activating topical anesthetic.Am J Ophthalmol, 1998,125(4):481
6 CHEN Su-qing(陈素青),REN Lei-ming(任雷鸣),HUANG Zhi-qiang(黄致强).Effects of tetrodotoxin on naloxone-precipitated withdrawal syndrome in morphine-dependent rats and mice(河豚毒素对大鼠和小鼠纳洛酮催促吗啡戒断症状的影响).Chin J Pharmacol Toxicol(中国药理学与毒理学杂志),2001,15(6):434
7 XU Ying(徐英),KU Bao-shan(库宝善),HU Gang(胡刚),et al.The study on analgesic effects of tetrodotoxin combined with morphine(河豚毒素与吗啡联合应用的协同作用研究).J Jiangsu Clin Med(江苏临床医学杂志),2001,5(5):361
8 DAI Xiu-mei(代秀梅),YU Li-ju(庾莉菊),ZHANG Qi-ming(张启明),et al.The prospect of tetrodotoxin on medicine exploitation(河豚毒素的医药开发前景).Drug Evaluation(药品评价),2008,5(5):230
9 CHEN Wei-zhen(陈唯真),ZHU Wei-hua(朱维华),YU Ru-ying(俞如英).HPLC determination of tetrodoxin and its stability(HPLC法测定河豚毒素的含量及稳定性).Chin J Pharm Anal(药物分析杂志),2004,24(1):41
10 WANG Jin(王瑾),YIN Li-hui(尹利辉).ICP-MS determination of the trace elements in Radix Codonopsis(ICP-MS法测定党参中微量元素含量).Chin J Pharm Anal(药物分析杂志),2008,28(10):1689
11 CHEN Qiu-sheng(陈秋生),CHENG Yi(程奕),MENG Zhao-fang( 孟兆芳),et al. Studies on simultaneous determination of multiple trace elements in Chinese Traditional Patent Medicine with inductively coupled plasma mass spectrometry(电感耦合等离子体质谱法同时测定中成药中多种微量元素的研究).Chin J Pharm Anal(药物分析杂志),2008,28(10):399
12 RE Zeng-cai dan(热增才旦),LIU Bin(刘斌),WANG Ying-feng(王英锋),et al.ICP-MS determination of the trace elements in manubzhithang(微波消解ICP-MS法测定藏药四味藏木香散中微量元素).Chin J Pharm Anal(药物分析杂志),2010,30(10):1852