阿片类化合物中氮氧化物杂质的定位鉴别

目的:建立阿片类化合物中主要杂质氮氧化物的鉴别溶液,解决国内缺少该类杂质对照品的问题。方法: 选择盐酸纳洛酮、磷酸可待因、盐酸氢可酮、蒂巴因、盐酸羟考酮5种代表性的阿片类化合物,采用溶剂溶解,双氧水加热破坏的方法得到氮氧化物的鉴别溶液,并采用UPLC-MS/MS法进行验证。结果: 经UPLC－MS/MS验证,基本确定破坏得到了5种阿片类化合物的氮氧化物鉴别溶液。结论: 该方法简便、准确,可推广应用于阿片类化合物中主要杂质氮氧化物的定位鉴别。

Objective: To establish the identification solutions of the main related N-oxide compounds for opioids to solve the problem of lack of N-oxide reference standards in our nation. Methods: Five selected typical opioid compounds were dissolved in the H₂O₂ solution with thermal destruction respectively,thus the oxidation stress treated solutions were obtained to be used as the identification solutions of related N-oxides reference after being verified by UPLC-MS/MS. Results: It was verified by UPLC-MS/MS that the oxidation stress treated opioids solutions could be successfully used as the identification solution for the five N-oxide compounds in opioid compounds. Conclusion: The method is simple and accurate,and its application can be promoted for location identification of main N-oxides in opioid compounds.

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