HPLC测定不同产地土茯苓中落新妇苷和黄杞苷含量

目的:采用HPLC法对不同产地的土茯苓中落新妇苷和黄杞苷的含量进行测定。方法: 采用Waters SUNfire ODS(4.6 mm×250 mm,5 μm)色谱柱,以甲醇-水-磷酸溶液(35:65:0.1) 为流动相,流速1.0 mL·min^-1,柱温35℃,检测波长为290 nm。结果: 落新妇苷和黄杞苷分别在0.051~1.02 μg和0.058~1.16 μg范围内内呈良好的线性关系,平均加样回收率(n=9)为99.23%和99.93%。结论: 本方法可用于土茯苓药材的质量控制;不同产地土茯苓中落新妇苷和黄杞苷的含量差异较大。

Objective: To determine astilbin and engeletin in Smilax glabra from different producing areas by HPLC. Methods: The samples were separated at 35℃ on a Waters SUNfire ODS column(4.6 mm×250 mm,5 μm)eluted with methanol-water-phosphoric acid (35:65:0.1) as the mobile phase.The flow rate was at 1.0 mL·min^-1 and the detection wavelength was 290 nm. Results: The calibration curves of astilbin and engeletin were linear over the ranges of 0.051-1.02 μg and 0.058-1.16 μg,the average recoveries (n=9) were 99.23% and 99.93%,respectively. Conclusion: The method can be used as a quality control method for Smilax glabra;contents of astilbin and engeletin in Smilax glabra from different producing areas are quite different.

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