组织工程支架材料聚乙醇酸的体外细胞贴壁性和降解性能研究

目的:选择可降解材料PGA作为研究对象,对其细胞贴壁性和体外及体内降解性能进行研究。方法: 通过将L929细胞与支架材料共培养的方法对PGA支架材料的细胞贴壁性进行研究。设计试验计算出细胞在材料上的粘附率,并且通过扫描电子显微镜观察了细胞在材料表面的贴壁形态。在体外降解试验中,分别在去离子水和磷酸盐缓冲液中,在37℃和70℃的条件下对材料的降解性能进行观察。用大鼠皮下包埋试验对PGA在大鼠皮下包埋后1周、4周、8周、12周这4个时间点的降解表现进行了观察。结果: 试验结果表明,共培养72 h后,扫描电镜观察到L929细胞在PGA材料上良好贴壁,细胞粘附率大于80%;降解试验表明PGA组织工程支架在动物体内12周内基本降解。结论: 体外细胞贴壁性和降解试验的数据表明PGA组织工程支架符合组织工程产品支架材料的需求,是一种较好的组织工程支架材料。

Objective: To study the cell compatibility and biodegrading property of a polyglycolic acid(PGA) scaffold both in vitro and in vivo. Method: Cell compatibility was evaluated by co-culturing L929 cells with the PGA scaffolds.Then,in vitro and in vivo degrading tests were performed to evaluate the degrading property.In the in vitro degrading test,degradation property was observed both in deionized water and phosphate buffer saline in 37℃ and 70℃.In the in vivo degrading test,the PGA scaffolds were subcutaneously implanted in the rats. Results: The results indicated that PGA scaffold provided a suitable surface and environment for cell adhesion,and it could gradually degrade both in vivo and in vitro. Conclusion: These results indicate that PGA provides a suitable material for tissue engineering.

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