HPLC法测定不同产地桂花中红景天苷和毛蕊花糖苷的含量

目的: 建立HPLC法测定不同产地桂花中红景天苷和毛蕊花糖苷的含量。 方法: 采用Agilent ZORBAX SB-C₁₈色谱柱(4.6 mm×250 mm,5 μm),以乙腈-0.1%磷酸水为流动相,梯度洗脱,流速1.0 mL·min^-1,检测波长220 nm,柱温为室温,进样量10 μL。 结果: 红景天苷和毛蕊花糖苷的质量浓度分别在1.06~3.89 μg·mL^-1(r=0.9995,n=6)、8.84~32.4 μg·mL^-1(r=0.9997,n=6)内与峰面积呈良好的线性关系;平均回收率分别为98.3%和98.4%, RSD分别为1.1%和0.65%。通过比较不同产地桂花样品的测定结果,可知不同产地桂花中红景天苷和毛蕊花糖苷的含量有着较大差别。 结论: 该方法为各产地桂花药材进一步开发利用提供质量控制依据。

Objective: To establish an HPLC method for simultaneous determination of salidroside and verbascoside in Osmanthus fragrans produced from different places. Methods: The chromatographic separation was performed on an Agilent ZORBAX SB-C₁₈ column(4.6 mm×250 mm,5 μm);the mobile phase was acetonitrile-0.1 % phosphoric acid solution with gradient elution at the flow rate of 1 mL·min^-1;the detection wavelength was 220 nm,the column temperature was room temperature,and the injection volume was 10 μL. Results: The linear ranges were 1.06-3.89 μg·mL^-1(r=0.9995,n=6)for salidroside and 8.84-32.42 μg·mL^-1 (r=0.9997,n=6)for verbascoside,respectively.The average recovery of salidroside was 98.3%,RSD was 1.1%;the verbascoside average recovery was 98.4%,RSD was 0.65%.From the results obtained in this study,the contents of salidroside and verbascoside had great difference in Osmanthus fragrans collected from different places of origin. Conclusion: The method provides reference for the quality control for further development of Osmanthus fragrans in different producing places.