目的:测定清肺止咳颗粒中木兰脂素、秦皮甲素、秦皮乙素的含量。方法:采用HPLC法,使用十八烷基硅烷键合硅胶色谱柱DiamonsiL C18(250 mm×4.6 mm,5 μm)。测定木兰脂素的流动相为乙腈-水(40∶60),流速1 mL·min-1,检测波长为278 nm;测定秦皮甲素、秦皮乙素的流动相为乙腈-0.1%磷酸溶液(8∶92),流速1.0 mL·min-1,检测波长为334 nm。结果:木兰脂素、秦皮甲素、秦皮乙素进样量分别在0.22~1.76 μg(r=0.9998)、0.102~0.306 μg(r=0.9997)、0.23~0.69 μg(r=0.9997)范围内线性关系良好;平均回收率为99.9%、97.7%、100.0%,RSD为1.4%、2.0%、1.8%。结论:所建立的分析方法可作为清肺止咳颗粒的质控标准。
Objective:To determine the content of magnolin,aesculin,aesculetin in Qingfeizhike Particles by HPLC.Methods:An HPLC method with a Diamonsil C18 column(250 mm×4.6 mm,5 μm) was used.For magnolin,the solution of acetonitrile-water (40∶60) was used as the mobile phase at a flow rate of 1.0 mL·min-1 and a detection wavelength of 278 nm;for aesculin and aesculetin,acetonitrile-0.1% phosphoric acid (8∶92) was used as the mobile phase at a flow rate of 1.0 mL·min-1 and a detection wavelength of 334 nm.Results:The linear relationships of magnolin,aesculin and aesculetin were good within the ranges of 0.22-1.76 μg (r=0.9998),0.102-0.306 μg(r=0.9997) and 0.23-0.69 μg (0.9997),respectively,and the average recovery rates were 99.9%,97.7% and 100.0%,respectively,with RSD of 1.4%,2.0% and 1.8%.Conclusion:The established method can be used for the quality control standard of Qingfeizhike Particles.
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