单因素试验结合响应面分析法优化杜仲最佳提取工艺

目的:以松脂醇二葡萄糖苷(pinoresinol diglucoside,PDG)含量为指标,研究杜仲最佳提取工艺,为大鼠经口给予杜仲提取物进行体内药代动力学研究提供供试品。方法:应用单因素试验结合响应面分析法(response surface methodology),对杜仲提取工艺进行优化,在单因素试验的基础上选取因素不同水平,根据中心组合(Box-Benhnken)试验设计原理,采用三因素三水平的响应面分析法确定最佳提取工艺。结果:以PDG含量为指标,杜仲最佳提取工艺条件为:提取方法为超声提取,提取溶剂为26%乙醇溶液,料液比1∶13,提取时间25 min,此条件下PDG含量的提取预测值为3.2809 mg·g^-1原药材。结论:超声提取法与中国药典(2010年版)中杜仲供试品溶液的制备方法比较,具有提取率高、能耗少、时间短等优点,为杜仲体内药代动力学研究奠定了基础。

Objective:To approach an optimal extraction process with the content of pinoresinol diglucoside(PDG) as an index for Eucommia ulmoides,and provide a basis for the in vivo pharmacokinetic studies in rats after oral administration of the Eucommia ulmoides extract.Methods:The single factor experiment was utilized combined with response surface methodology to optimize the extraction process of Eucommia ulmoides.On the basis of single factor experiments,different optimum conditions for the extraction of PDG from Eucommia ulmoides were selected,and the method of response surface analysis with 3 factors and 3 levels was adopted according to the Box-Benhnken center-united experimental design principles.Results:The results showed that the optimum conditions for extraction of PDG from Eucommia ulmoides were as follows:Eucommia ulmoides was extracted using 26% of ethanol solution with the ratio of material to solvent at 1∶13 for 25 min by ultra sonification,under which the extraction predictive value of PDG was 3.2809 mg·g^-1.Conclusion:Compared with the method described in Chinese Pharmacopoeia(version 2010),this method has advantages of higher extraction yield,lower energy consumption,and shorter extraction time,which will lay a good foundation for the subsequent in vivo pharmacokinetic study of Eucommia ulmoides.