双波长HPLC法同时测定败酱草中原儿茶酸、绿原酸及咖啡酸的含量

目的: 建立双波长高效液相色谱法同时测定败酱草中原儿茶酸、绿原酸及咖啡酸含量的方法。方法: 用Odyssil C₁₈色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.1%磷酸(10∶90),流速为1.0 mL·min^-1,检测波长为260、327 nm,柱温为25 ℃,进样量为5 μL。 结果: 原儿茶酸进样量在3.42×10^-2~3.42×10^-1 μg 范围内与峰面积呈现良好的线性关系(r =0.9997),平均加样回收率为101.6％,RSD为1.9％;绿原酸进样量在4.66×10^-2~4.66×10^-1 μg 范围内与峰面积呈现良好的线性关系(r =0.9999),平均加样回收率为99.2％,RSD为1.7％;咖啡酸进样量在1.77×10^-2~2.21×10^-1 μg范围内与峰面积呈现良好的线性关系(r =0.9996),平均加样回收率为98.6％,RSD为2.3％。结论: 该方法简便、快速、具有良好的重现性,可用于败酱草中原儿茶酸、绿原酸和咖啡酸的含量测定。

Objective: To develop a dual-wavelength HPLC method for the determination of protocatechuic acid,chlorogenic acid and caffeic acid contents in Herba Patriniae.Methods:The Odyssil C₁₈ column(250 mm×4.6 mm,5 μm)was used with a mobile phase of acetonitrile -0.1% phosphate acid at 1.0 mL·min^-1,and the detection wavelengths were set at 260 nm and 327 nm.The column temperature was controlled at 25 ℃ and the injection volume was 5 μL.Results:The method displayed good linearity over the concentration ranges of 3.42×10^-2-3.42×10^-1 μg(r =0.9997)for protocatechuic acid,4.66×10^-2-4.66×10^-1 μg(r =0.9999)for chlorogenic acid and 1.77×10^-2-2.21×10^-1 μg(r =0.9996)for caffeic acid,respectively.The average recoveries of protocatechuic acid,chlorogenic acid and caffeic acid were 101.6％,99.2% and 98.6%,with the RSD of 1.9%,1.7%,2.3%,respectively.Conclusion:The results indicated that the present RP-HPLC method is simple,accurate and reproducible,which is suitable for the quality control of protocatechuic acid,chlorogenic acid and caffeic acid in Herba Patriniae.