HPLC法同时测定复方夏枯草搽剂中3个有效成分的含量

目的: 建立复方夏枯草搽剂中盐酸小檗碱和补骨脂素、异补骨脂素的HPLC含量测定方法。 方法: 采用Zorbax SB-C₁₈色谱柱(250 mm×4.6 mm,5 μm),以甲醇-0.1%磷酸溶液(45∶55)为流动相,流速1.1 mL·min^-1,柱温40 ℃,检测波长265 nm。 结果: 盐酸小檗碱和补骨脂素、异补骨脂素线性范围分别为21.0~315.0 μg·mL^-1(r=0.9994)、14.4~216.0 μg·mL^-1(r=0.9996)、17.3~260.0 μg·mL^-1(r=0.9997)。回收率(n=6)分别为97.9%、98.5%、98.9%;RSD分别为1.5%、1.3%、0.9%。 结论: 该方法可用于复方夏枯草搽剂的质量控制。

Objective: To establish an HPLC method for the determination of berberine hydrochloride,psoralen and isopsoralen in compound Xiakucao liniment. Methods: A Zorbax SB-C₁₈ column (250 mm×4.6 mm,5 μm) was used,the mobile phase consisted of methanol-0.1% phosphoric acid(45∶55) at the flow rate of 1.1 mL·min^-1,the column temperature was 40 ℃,and the detection wavelength was set at 265 nm. Results: The linear ranges of berberine hydrochloride,psoralen and isopsoralen were 21.0-315.0 μg·mL^-1(r=0.9994),14.4-216.0 μg·mL^-1(r=0.9996),17.3-260.0 μg·mL^-1(r=0.9997),respectively;the average recoveries(n=6) was 97.9%,98.5%,98.9% with RSDs of 1.5%,1.3%,0.9%,respectively. Conclusion: The method can be used for quality control of compound Xiakucao liniment.

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