黑草药材HPLC指纹图谱的研究

目的:以不同产地的黑草为研究对象,建立黑草药材的HPLC指纹图谱,为科学评价和有效控制黑草药材的质量提供科学依据。 方法:以木犀草素成分为参照峰,采用HPLC法建立黑草药材的指纹图谱;色谱柱:Agilent Extend-C₁₈(250 mm× 4.6 mm,5 μm),流动相:乙腈与0.7%冰醋酸,梯度洗脱;流速:1.0 mL·min^-1;检测波长:350 nm;柱温:35℃;进样量:10 μL。 结果:10批次黑草药材中共有12个指纹图谱峰,经相似度计算,整体相似性好。 结论:本文建立的指纹图谱有较强的针对性,为黑草药材的研究以及制定黑草药材的质量控制标准提供科学依据。

Objective: To establish the HPLC fingerprint of the herb of Buchnera cruciata Hamilt.from different regions,and provide the scientific foundation for quality evaluation and quality control of the crude drug. Methods: HPLC method was adopted and luteolin was used as an internal standard compound to build the HPLC fingerprint.The Agilent Extend-C₁₈ column(250 mm× 4.6 mm,5 μm)was used,UV detection wavelength was set at 350 nm,and the column temperature was 35℃.The mobile phase was a linear gradient of acetonitrile and 0.7% glacial acetic acid at the flow rate of 1.0 mL·min^-1. Results: Twelve peaks were identified in HPLC fingerprint of ten batches samples of B.cruciata,and the overall similarity was good according to the similarity calculation. Conclusion: The fingerprint was specific and the established method can be used for research and quality control of the crude drug of B.cruciata.

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