RP-HPLC法同时测定枸杞子中5个酚酸类成分的含量

目的:建立RP-HPLC法同时测定不同产地、同产地不同种之间、同产地不同野生与人工栽培枸杞子中原儿茶醛、儿茶素、表儿茶素、咖啡酸和阿魏酸的含量。 方法:使用快速溶剂萃取仪(ASE 350)用50%甲醇水溶液提取出酚酸类成分,采用Spursil C₁₈-EP(250 mm ×4.6 mm,5 μm)色谱柱;流动相为甲醇-0.04%冰乙酸水溶液梯度洗脱,流速1.0 mL·min^-1,检测波长280 nm,柱温30℃。 结果:原儿茶醛、儿茶素、表儿茶素、咖啡酸和阿魏酸的进样浓度分别在24.05~601.2 mg·L^-1、4.396~109.9 mg·L^-1、17.06~426.6 mg·L^-1、0.7104~17.76 mg·L^-1、1.085~27.12 mg·L^-1范围内与峰面积呈良好的线性(r=0.9995~0.9998);平均加样回收率(n=6)分别为97.4%、97.7%、98.7%、95.5%、96.3%。 结论:本方法快速、准确,重现性好,适用于枸杞子中5个酚酸类成分的含量测定,可为枸杞的质量控制提供依据。

Objective: To establish a RP-HPLC method for simultaneous determination of protocatechuic aldehyde,catechin,epicatechin,caffeic acid and ferulic acid in Lycii Fructus from different habitats,different species in same habitats,different wild and artificial cultivation in same habitats. Methods: The five phenolic acids were extracted using accelerated solvent extraction(ASE 350)with 50% methanol-water.The chromatographic method was carried out on a Spursil C₁₈-EP column(250 mm ×4.6 mm,5 μm)with gradient elution using mobile phase consisting of methanol-0.04% acetic acid;The flow rate was 1.0 mL·min^-1with the column temperature at 30℃,and the absorbance was monitored at 280 nm. Results: The linear response ranges of protocatechuic aldehyde,catechin,epicatechin,caffeic acid and ferulic acid were 24.05-601.2 mg·L^-1,4.396-109.9 mg·L^-1,17.06-426.6 mg·L^-1,0.7104-17.76 mg·L^-1,and 1.085-27.12 mg·L^-1(r=0.9995-0.9998),respectively;The average recoveries(n=6)were 97.4%,97.7%,98.7%,95.5%,and 96.3%,respectively. Conclusion: The established method has adequate accuracy and selectivity,which is suitable for the determination of five phenolic acids components in Lycii Fructus.Moreover,it gives additional evidence for the quality evaluation of Lycium barbarum L.