RP-HPLC法同时测定不同产地甘草中9个主要成分的含量

目的: 建立同时测定甘草药材中芹糖基甘草苷、甘草苷、芹糖基异甘草苷、异甘草苷、甘草查尔酮B、甘草素、刺甘草查尔酮、异甘草素和甘草酸含量的高效液相色谱方法。 方法: 采用Diamonsil^TM C₁₈色谱柱(250 mm×4.6 mm,5 μm),以乙腈-0.05%磷酸水溶液为流动相,梯度洗脱,流速为0.8 mL·min^-1,检测波长为276 nm(0~20 min)、360 nm(20~30 min)、276 nm(30~35 min)、370 nm(35~48 min)、248 nm(48~55 min),柱温为40℃。 结果: 芹糖基甘草苷、甘草苷、芹糖基异甘草苷、异甘草苷、甘草查尔酮B、甘草素、刺甘草查尔酮、异甘草素和甘草酸的浓度分别在13.1-262 μg·mL^-1(r=0.9994)、8.3-100 μg·mL^-1(r=0.9997)、5.2~104 μg·mL^-1(r=0.9997)、0.96~11.5 μg·mL^-1(r=0.9995)、0.31~10.00 μg·mL^-1(r=0.9999)、1.11~22.2 μg·mL^-1(r=0.9991)、0.23~7.25 μg·mL^-1(r=0.9998)、0.52~10.40 μg·mL^-1(r=0.9996)、25.25~808.0 μg·mL^-1(r=0.9995)与各自峰面积值呈良好的线性关系;平均回收率(n=6)分别为103.6%、104.5%、104.9%、100.4%、102.2%、101.5%、100.4%、98.5%、101.7%。 结论: 本方法简便,重复性好,可以更好地用于甘草药材的质量控制。

Objective: To develop an HPLC method for simultaneous determination of 9 main constituents (liquiritin apioside, liquiritin, isoliquiritin apioside, isoliquiritin, licochalcone B, liquiritigenin, echinatin, isoliquiritigenin, glycyrrhizic acid) in the roots and rhizomes of Glycyrrhiza uralensis Fisch. Methods: The analyses were performed on a Diamonsil^TM ODS C₁₈ column(250 mm×4.6 mm,5 μm)with the gradient elution of acetonitrile-0.05% phosphoric acid aqueous at a flow rate of 0.8 mL·min^-1.The detection wavelength was set at 276 nm(0-20 min),360 nm(20-30 min),276 nm(30-35 min),370 nm(35-48 min),248 nm(48-55 min)and the column temperature was at 40℃. Results: Good linearity was obtained for liquiritin apioside,liquiritin,isoliquiritin apioside,isoliquiritin,licochalcone B,liquiritigenin,echinatin,isoliquiritigenin,and glycyrrhizic acid in ranges of 13.1-262 μg·mL^-1(r=0.9994),8.3-100 μg·mL^-1(r=0.9997),5.2-104 μg·mL^-1(r=0.9997),0.96-11.5 μg·mL^-1(r=0.9995),0.31-10.00 μg·mL^-1(r=0.9999),1.11-22.2 μg·mL^-1(r=0.9991),0.23-7.25 μg·mL^-1(r=0.9998),0.52-10.40 μg·mL^-1(r=0.9996)and 25.25-808.0 μg·mL^-1(r=0.9995),respectively.The average recovery(n=6)of the nine constitutions was 103.6%、104.5%、104.9%、100.4%、102.2%、101.5%、100.4%、98.5%、101.7%,respectively. Conclusion: The developed HPLC method is simple and repeatable,which is helpful for the quality control of the roots and rhizomes of G. uralensis.