HPLC-DAD-ELSD法同时测定黄芪中5个成分的含量

目的: 建立HPLC-DAD-ELSD法同时测定黄芪药材中黄酮类和皂苷类成分的方法,比较15批不同产地的黄芪药材中各有效成分的含量。 方法: 采用HPLC-DAD-ELSD联用法,乙腈和水不同比例梯度洗脱,流速1 mL·min^-1,检测波长254 nm;蒸发光散射检测器(ELSD)漂移管温度112.8℃,载气流速3.2 L·min^-1,同时测定黄芪药材中毛蕊异黄酮、芒柄花素、黄芪甲苷(黄芪皂苷Ⅳ)、黄芪皂苷Ⅱ和黄芪皂苷Ⅲ的含量。 结果: 通过1次进样,同时测定了2个黄酮类和3个皂苷类等成分的含量,且线性关系良好(r=0.9992~0.9999)。平均回收率为99.1%~100.9%。 结论: 该方法简便,重复性好,可用于黄芪药材中黄酮类和皂苷类成分的同时测定,也可用于黄芪药材的质量控制。

Objective: To establish a quantitative method of two major active isoflavonids and three main saponins in Radix Astragali by high-performance liquid chromatography coupled with diode arry and evaporative light scattering detectors(HPLC-DAD-ELSD),and compare the contents of calycosin,formononetin and astragaloside Ⅳ,Ⅱ,Ⅲ among 15 batches from different habitats. Methods: HPLC-DAD-ELSD was conducted on a Spursil ^TMC₁₈ column(5 μm,250 mm×4.6 mm)with gradient elution,the mobile phase was ACN and H₂O,the temperature of drift tube was 112.8℃ and the nebulizing gas flow rate was 3.2 L·min^-1. Results: Five compounds were simultaneously detected with one injection,good linear relationships were found(r=0.9992-0.9999),and the average recovery rates were 99.13%-100.94%. Conclusions: The HPLC-DAD-ELSD method is simple and reproducible,which can be used as principles of quantitative analysis.

1 ChP(中国药典).2010.VolⅠ(一部):212
2 HU Fang-di(胡芳弟),FENG Shi-lan(封士兰),ZHAO Jian-xiong(赵健雄),et al.HPLC fingerprints of Astragalus and the determination of main components(黄芪的高效液相色谱指纹图谱及主成分含量测定).J Chin Med Mater(中药材),2004,27(11):831
3 SHI Zi-yi(石子仪),BAO Zhong(鲍忠),JIANG Yong(姜勇),et al.Determination of calycosin-7-O-β-D- glucoside and formononetin of Astragalus from different sources(不同来源黄芪药材中毛蕊异黄酮葡萄糖苷和芒柄花素的定量分析).Chin J Chin Mater Med(中国中药杂志),2007,32(5):779
4 JI Song-gang(纪松岗),LI Xiang(李翔),LOU Zi-yang(娄子洋).Determination of calycosin-7-O-β-D- glucoside and formononetin of Astragalus by HPLC(高效液相色谱法测定黄芪中毛蕊异黄酮苷和芒柄花素的含量).Acad J Second Mil Med Univ(第二军医大学学报),2006,27(1):814
5 YAO Xue-lian(姚雪莲),PEI Cai-yun(裴彩云),WANG Zong-quan(王宗权).Determination of calycosin-7-O-β-D- glucoside and formononetin in crude drugs and slices of Astragalus from different habitats and gathering periods(不同产地、不同采收期黄芪药材及饮片中毛蕊异黄酮葡萄糖苷及芒柄花素含量测定).Chin J Pharm Anal(药物分析杂志),2010,30(5):797
6 CHENG Hai-yan(程海燕),CHEN Xiao-hui(陈晓辉),LI Qing(李清),et al.RP-HPLC simultaneous determ ination of six flavonoids in Radix Astragali(RP-HPLC法同时测定黄芪药材中6个黄酮类成分的含量).Chin J Pharm Anal(药物分析杂志),2009,29(7):1115
7 ZHAO Qiang-qiang(赵强强),HAN Li(韩丽),OU Shui-ping(欧水平).Determination of astragaloside I,II and acetytastragalosideI in astragalus extract by HPLC-ELSD(HPLC-ELSD法测定黄芪提取物中黄芪皂苷Ⅰ,Ⅱ及乙酰黄芪皂苷).Chin J Exp Tradit Med Form(中国实验方剂学杂志),2011,17(11):56
8 WANG Hong(汪红),WANG Qiang(王强).Determination of Astragaloside Ⅳ,Ⅲ,Ⅰin Radix Astragali and its injection by HPLC-ELSD(HPLC-ELSD法测定黄芪及其注射液中黄芪皂苷Ⅳ,Ⅲ,Ⅰ的含量).Chin Pharm J (中国药学杂志),2002,37(4):298
9 WANG Ju-tao(王举涛),XU Wen-fang(徐文方),LIU Jin-qi(刘金旗).Determination of astragaloside Ⅳ in Radix Astragali by HPLC-ELSD(HPLC-ELSD法测定黄芪中黄芪甲苷的含量).Anhui Pharm(安徽药学),2009,10(1):28
10 ZHAO Ling-zhi(赵灵芝),ZHUN Dan-ni(朱丹妮),YAN Yong-qing(严永清).Determination of astragaloside Ⅳ in Radix Astragali by HPLC-ELSD(HPLC-ELSD法测定黄芪中黄芪甲苷的含量).Chin J Pharm Anal(药物分析杂志),1999,19(6):403
11 MA Ling(马玲),WANG Kun(王坤),ZHANG Lu-yan(张芦艳),et al.马HPLC-ELSD Determination, of astragalosiden in Radix Astragali of different ages(HPLC-ELSD 法测定不同生长年限黄芪中黄芪甲苷的含量).Chin J Pharm Anal(药物分析杂志),2006,26(12):1869
12 WANG Zong-quan(王宗权),JIA Ji-ming(贾继明),SONG Jian(宋剑),et al.Determination of astragaloside Ⅰ,astragaloside Ⅱ and astragaloside Ⅳ in Radix Astragali from various habitats(不同产地黄芪中黄芪皂苷Ⅰ、黄芪皂苷Ⅱ和黄芪皂苷Ⅳ含量测定).Chin J Pharm Anal(药物分析杂志),2010,30(7):1191