GC法同时测定明目上清片中4个成分的含量

目的: 建立同时分析测定明目上清片中4个活性成分(柠檬烯、薄荷酮、薄荷脑和胡薄荷酮)的方法。方法: 采用GC法,色谱柱为DB-1701毛细管色谱柱(30 m×0.3 mm,0.25 μm),柱温采用程序升温(80 ℃保持1 min,5 ℃·min^-1上升至120 ℃,保持1 min,再以50 ℃·min^-1的速率升温至220 ℃,保持2 min);进样口温度:180 ℃;检测器:FID;检测器温度:250 ℃。结果: 柠檬烯、薄荷酮、薄荷脑和胡薄荷酮线性范围分别为0.0103～0.507 mg·mL^-1(r=0.9997)、0.0105～0.501 mg·mL^-1(r=0.9999)、0.0082～0.412 mg·mL^-1(r=0.9991)、0.0085～0.404 mg·mL^-1(r=0.9998);平均加样回收率(n=6)分别为99.1%、97.6%、98.7%、98.3%,RSD分别为1.2%、1.8%、1.4%、1.5%。结论: 该方法可用于明目上清片的质量检测。

Objective: To establish a method for simultaneous determination of four active ingredients (limonene, menthone, menthol, pulegone) in Mingmushangqing tablets. Methods: The determination was performed on a DB-1701 column (30 m×0.3 mm, 0.25 μm).The column temperature was maintained at 80 ℃ for 1 min, and programmed to 120 ℃ at the rate of 5 ℃·min^-1, then held for 1 min.After that the temperature was elevated to 220 ℃ at the rate of 50 ℃·min^-1.The FID was used as detector, the temperatures of the injection and the detector were set at 180 ℃ and 250 ℃ and maintained for 2 min. Results: The calibration curves were linear in the ranges of 0.0103-0.507 mg·mL^-1 for limonene (r=0.9997), 0.0105-0.501 mg·mL^-1 for menthone (r=0.9999), 0.0082-0.412 mg·mL^-1 for menthol (r=0.9991), and 0.0085-0.404 mg·mL^-1 for pulegone (r=0.9998).The average recoveries (n=6) were 99.1%, 97.6%, 98.7% and 98.3%;RSDs were 1.2%, 1.8%, 1.4% and 1.5%, respectively. Conclusion: The method can be used for quality control of Mingmushangqing tablets.

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