RP-HPLC法同时测定皮尔康胶囊中3个成分的含量

目的: 建立RP-HPLC同时测定皮尔康胶囊中龙胆苦苷、黄芩苷、花椒毒素含量的分析方法。方法: 采用DIONEX Acclaim C₁₈色谱柱(250 mm×4.6 mm,5 μm),以乙腈-水(14:86)为流动相,流速1 mL·min^-1,检测波长270 nm,柱温30 ℃。结果: 龙胆苦苷、黄芩苷、花椒毒素进样量分别在0.10～1.5 μg、0.032～0.47 μg、0.0032～0.048 μg范围内与峰面积呈良好的线性关系(r=0.9996～0.9999,n=5),平均回收率(n=6)分别为99.8%(RSD=0.76%)、100.2%(RSD=1.8%)、99.8%(RSD=1.9%)。结论: 本法适用于皮尔康胶囊中龙胆苦苷、黄芩苷、花椒毒素3个成分的同时测定。

Objective: To develop a RP-HPLC method for the simultaneous determination of gentiamarin, baicalin and xanthotoxin in Pierkang capsules. Methods: A DIONEX Acclaim C₁₈column (250 mm×4.6 mm, 5 μm) was used.The mobile phase was acetonitile-water (14:86) at the flow rate of 1.0 mL·min^-1, the detection wavelength was set at 270 nm, and the column temperature was 30 ℃. Results: There was a good linear relationship between the quantity and peak area in the range of 0.10-1.5 μg, 0.032-0.47 μg, 0.0032-0.048 μg for gentiamarin, baicalin and xanthotoxin (r=0.9996-0.9999, n=5), respectively;the average recoveries of gentiamarin, baicalin and xanthotoxin were 99.8%(RSD=0.76%), 100.2%(RSD=1.8%), and 99.8%(RSD=1.9%), respectively. Conclusion: The method is suitable for simultaneous determination of gentiamarin, baicalin and xanthotoxin in Pierkang capsules.

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