HPLC波长切换法同时测定大黄、牡丹皮药对提取物中11个成分的含量

Objective: To develop an HPLC method for determination of 11 indicative components (gallic acid, hydroxyl-paeoniflorin, paeoniflorin, 1, 2, 3, 4, 6-pentagalloylglucose, paeonol, benzoyl-paeoniflorin, aloe-emodin, rhein, emodin, chrysophanol, physcion) in Rhei Radix et Rhizoma and Moutan Cortex. Methods: The Phenomsil ODS column (250 mm×4.6 mm, 5 μm) was adopted;the mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B)with gradient elution at a flow rate of 1.0 mL·min^-1, the detection wavelength was 267 nm (0-20 min, gallic acid), 258 nm (20-30 min, hydroxyl-paeoniflorin), 230 nm (30-50 min, paeoniflorin), 274 nm (50-80 min, 1, 2, 3, 4, 6-pentagalloylglucose, paeonol), 230 nm (80-90 min, benzoyl-paeoniflorin), 254 nm (90-125 min, aloe-emodin, rhein, emodin, chrysophanol, physcion), and the column temperature was set at 30 ℃. Results: The content of 11 indicative components in Rhei Radix et Rhizoma and Moutan Cortex was stable.The method had a good linearity in the ranges of 0.365-3.65 μg (r=0.9991) for gallic acid, 0.0349-0.349 μg(r=0.9995)for hydroxyl-paeoniflorin, 0.106-1.06 μg (r=0.9993) for paeoniflorin, 0.215-2.15 μg (r=0.9996) for 1, 2, 3, 4, 6-pentagalloylglucose, 0.259-2.59 μg (r=0.9994) for paeonol, 0.0758-0.758 μg (r=0.9993) for benzoyl-paeoniflorin, 0.0846-0.846 μg (r=0.9993) for aloe-emodin, 0.0581-0.581 μg (r=0.9993) for rhein, 0.109-1.09 μg (r=0.9992) for emodin, 0.164-1.64 μg (r=0.9991) for chrysophanol, 0.0424-0.424 μg (r=0.9995) for physcion.The average recoveries (n=5) were 100.1%, 98.4%, 99.5%, 99.3%, 99.4%, 98.8%, 99.5%, 99.0%, 99.1%, 98.8%, 99.1%, respectively. Conclusion: This method can be used for quality control of Rhei Radix et Rhizoma and Moutan Cortex.

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