GC-MS和平滑预处理及SFA法用于茵陈挥发性成分的分析

目的: 研究茵陈的挥发性成分。方法: 利用水蒸气蒸馏法提取茵陈挥发油,用GC-MS检测。重叠色谱峰用粗糙惩罚平滑法处理后,用子窗口因子分析法(SFA)分辨,从而获得每一组分的纯色谱和质谱,依靠每一组分纯质谱在NIST质谱库进行相似性检索而定性分析,用总体积积分法定量分析。结果: 共分辨出67个色谱峰,鉴定出48个化学成分,占总含量的89.03%。主要组分为n-十六烷酸、9,12,15-十八碳三烯酸、镰叶芹醇、反式-Z-α-环氧红没药烯和大牻牛儿烯D。结论: 本法不仅可使鉴定的化合物数目增加,而且也提高了定性准确度,该法能用于茵陈的进一步开发和质量控制。

Objective: To study the volatile chemical constituents in the dried shoots of Artemisia capillaris Thunb. Methods: The essential oil was extracted by steam distillation.The chemical compositions were detected by GC-MS.After the overlapping of chromatographic peaks, which were first treated with roughness penalty smoothing method, were resolved into pure chromatograms and spectra with subwindow factor analysis method (SFA), qualitative analysis was performed by similarity search in NIST library with the obtained pure mass spectrum of each component and the quantitative results were obtained by overall volume integration. Results: A total of 67 chromatographic peaks were separated, and 48 chemical components in the essential oil of the dried shoots of Artemisia capillaris Thunb.were identified, which accounted for 89.03% of the total content.The main components were n-hexadecanoic acid, 9, 12, 15-octadecatrienoic acid, Artemisia capillaris falcarinol, trans-Z-alpha-bisabolene epoxide and germacrene D. Conclusions: Not only more components were identified by the established method, but also the accuracy of the qualitative analysis of components was improved.The established method can be used for further development and quality control of Artemisia capillaris Thunb.

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