HPLC法同时测定山玫胶囊中8个成分的含量

目的: 建立同时测定山玫胶囊中没食子酸、绿原酸、牡荆素葡萄糖苷、牡荆素鼠李糖苷、芦丁、牡荆素、金丝桃苷和槲皮素含量的高效液相色谱方法。 方法: 采用Agilent ZORBAX SB-C₁₈色谱柱(4.6 mm×250 mm,5 μm),以0.5%甲酸水溶液(A)-乙腈(B)-甲醇(C)-四氢呋喃(D)为流动相梯度洗脱,流速1.0 mL·min^-1,检测波长260 nm、参比波长360 nm(0~15 min),检测波长370 nm、参比波长430 nm(15~65 min),柱温30℃。 结果: 没食子酸、绿原酸、牡荆素葡萄糖苷、牡荆素鼠李糖苷、芦丁、牡荆素、金丝桃苷和槲皮素的线性范围分别为9.38~300,11.2~360,18.7~600,31.8~1020,1.31~42.0,7.50~240,9.00~288,0.563~18.0 μg·mL^-1;r分别为0.9999,0.9995,0.9999,0.9999,0.9998,0.9998,0.9998,0.9999。平均加样回收率(n=6)分别为99.5%(RSD=1.6%),99.0%(RSD=1.2%),99.8%(RSD=1.7%),99.8%(RSD=1.3%),100.5%(RSD=1.8%),99.6%(RSD=1.5%),99.9%(RSD=2.0%),100.6%(RSD=1.1%)。 结论: 该分析方法准确可靠,重复性好,为更好地控制山玫胶囊内在质量提供科学依据。

Objective: To develop an HPLC method for simultaneous determination of gallic acid,chlorogenic acid,glucosyl-vitexin,vitexin-2″-O-rhamnoside,rutin,vitexin,hyperoside and quercetin in compound Shanmei Capsules. Methods: The separation was carried out on an Agilent ZORBAX SB-C₁₈ column(4.6 mm×250 mm,5 μm) with 0.5% formic acid(A)-acetonitrile(B)-methanol(C)-tetrahydrofuran(D) as the mobile phase in a gradient elution mode.The flow rate was 1.0 mL·min^-1 at 0~15 min,the detection wavelength was 260 nm with the reference wavelength of 360 nm; at 15~65 min,the detection wavelength was 370 nm,the reference wavelength was 430 nm.The column temperature was 30℃. Results: The linear ranges of gallic acid,chlorogenic acid,glucosyl-vitexin,vitexin-2″-O-rhamnosid,rutin,vitexin,hyperoside and quercetin were 9.38-300 μg·mL^-1(r=0.9999),11.2-360 μg·mL^-1(r=0.9995),18.7-600 μg·mL^-1(r=0.9999),31.8-1020 μg·mL^-1(r=0.9999),1.31-42.0 μg·mL^-1(r=0.9998),7.50-240 μg·mL^-1(r=0.9998), 9.00-288 μg·mL^-1(r=0.9998),0.563-18.0 μg·mL^-1(r=0.9999),respectively.The average recovery rates of them were 99.5%(RSD=1.6%),99.0%(RSD=1.2%),99.8%(RSD=1.7%),99.8%(RSD=1.3%),100.5%(RSD=1.8%),99.6%(RSD=1.5%),99.9%(RSD=2.0%),and 100.6%(RSD=1.1%),respectively. Conclusion: This method provides a scientific basis for the quality control of compound Shanmei Capsules.