高效液相色谱法测定暴马子皮药材中2个成分的含量

目的: 建立测定暴马子皮药材中2个成分(紫丁香苷、sinapylaldehyde 4-O-β-D-glucopyranoside)的高效液相色谱分析方法。 方法: 采用Phenomenex Luna C₁₈(4.6 mm×250 mm,5 μm)色谱柱,流动相为甲醇(B)-0.1%醋酸水溶液(A),梯度洗脱,流速1.0 mL·min^-1,DAD检测器,检测波长:284 nm;柱温25 ℃。 结果: 紫丁香苷、sinapylaldehyde 4-O-β-D-glucopyranoside的线性范围分别为0.2~0.8 μg(r=0.9995)和0.04~0.16 μg(r=0.9993),平均加样回收率(n=6)分别为99.9%(RSD=2.1%)和99.5%(RSD=2.9%)。 结论: 不同产地暴马子皮药材中所含紫丁香苷、sinapylaldehyde 4-O-β-D-glucopyranoside的含量存在显著性差异。本方法操作简便,结果准确,重复性好,可用于中药暴马子皮药材的质量控制。

Objective: To develop an HPLC method for the determination of syringin and sinapyl aldehyde 4-O-β-D-glucopyranoside in Syringae Cortex. Methods: The separation was performed on a Phenomenex Luna C₁₈ (4.6 mm×25 0 mm,5 μm) column.The mobile phase was composed of methanol and water solvent containing 0.1% acetic acid with gradient elution.The flow rate was 1.0 mL·min^-1 and the detection wavelength was 284 nm.The column temperature was set at 25 ℃. Results: The calibration curves were linear in the ranges of 0.2-0.8 μg for syringin(r=0.9995) and 0.04-0.16 μg for sinapyl aldehyde 4-O-β-D-glucopyranoside(r=0.9993).The average recoveries(n=6) of syringin and sinapyl aldehyde 4-O-β-D-glucopyranoside were 99.9%(RSD=2.1%) and 99.5%(RSD=2.9%),respectively. Conclusion: The yields of the two components in different origins exhibit significant differences.This method is simple,accurate and repeatable,which can be used for the quality control of Syringae Cortex.