HPLC法测定地夫可特的含量及有关物质
HPLC determination of deflazacort and its related substances
分类号：
出版年·卷·期（页码）：2012,32 (9):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的:建立测定地夫可特含量及有关物质的高效液相色谱方法。方法:采用VP-ODS色谱柱(250 mm×4.6 mm,5 μm),以水-乙腈(60:40)为流动相,流速1.0 mL·min-1,检测波长244 nm。结果:在上述色谱条件下,各中间体及降解产物均可与地夫可特主峰良好分离。地夫可特与有关物质测定的线性范围分别为50.2~201.0 (] 结论:本文方法简便,可用于测定地夫可特的含量及有关物质。
-----英文摘要：---------------------------------------------------------------------------------------
Objective:To establish a RP-HPLC method for content determination of deflazacort and its related substances. Methods:The separation was performed on a Shimadzu VP-ODS column(250 mm×4.6 mm,5 μm)with the mobile phase consisting of water-acetontrile(60:40)at a flow rate of 1.0 mL·min-1,and the detection wavelength was set at 244 nm. Results:An excellent separation was achieved for deflazacort and its related substances.The linear ranges of deflazacort and its related substances were 50.2-201.0 (] Conclusions:The method is simple and can be successfully applied in the determination of deflazacort and its related substances.
-----参考文献：---------------------------------------------------------------------------------------
1 Di-Munno O,Mazzantini M,Milani S,et al.Clinical equivalence between deflazacort oral drops and tablets in active rheumatoid arthritis.Clin Rheumatol,1999,18(2):140
2 Gonzalez-Perez O,Luquin S,Garcia-Estrada J,et al.Deflazacort: A glucocorticoid with few metabolic adverse effects but important immunosuppressive activity.Adv Ther,2007,24(5):1052
3 Correa GM,Belle LP,Bajerski L,et al.Development and validation of a reversed-phase HPLC method for the determination of deflazacort in pharmaceutical dosage forms.Chromatographia,2007,61:591
4 EP 7.0.2010.2787
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