HPLC-ELSD测定酸枣仁汤不同配伍情况下酸枣仁皂苷A和B的含量

目的:建立HPLC-ELSD测定酸枣仁汤不同配伍情况下各组方中酸枣仁皂苷A和B含量的方法。方法:采用Grace Brava C₁₈-BDS(250 mm×4.6 mm,5 μm)色谱柱,以乙腈(A)-水(B)为流动相进行梯度洗脱,流速1.0 mL·min^-1,柱温25℃;ELSD参数:漂移管温度100℃,载气流速2.9 L·min^-1。结果:酸枣仁皂苷A和B浓度分别在49.50~900.0 μg·mL^-1和20.40~407.2 μg·mL^-1范围内呈良好的线性关系;平均加样回收率(n= 6)分别为101.9%(RSD=2.3%)和100.7%(RSD=3.0%)。结论:酸枣仁与其他药味配伍后,酸枣仁汤中酸枣仁皂苷A和B的含量发生一定变化,其他药味的存在不同程度地影响其溶出。

Objective:To establish an HPLC-ELSD method for determination of jujubosides A and B in different compatibility of Suanzaoren decoction. Methods:The assay was performed on a Grace Brava BDS C₁₈(250 mm×4.6 mm,5 μm) column by a gradient elution program with acetonitrile-water as mobile phase at a flow rate of 1.0 mL·min^-1,and the column temperature was 25℃.The evaporator tube temperature of ELSD was set at 100℃ with the nebulizing gas flow rate of 2.9 L·min^-1. Results: The linear ranges of jujubosides A and B were 49.50-900.0 μg·mL^-1 and 20.40-407.2 μg·mL^-1,and the average recoveries (n=6) were 101.9% (RSD=2.3%) and 100.7% (RSD=3.0%),respectively Conclusions:When Ziziphi Spinosae Semen was combined with different herbs in Suanzaoren decoction,the contents of jujubosides A and B were changed.For this reason,the other herbs in decoction would evidently affect the amounts of jujubosides A and B released from Suanzaoren decoction.

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